Bakht P, Ijaz M, Iqbal M Z, Aslam H B, Rehman A
Ph.D. Student in Veterinary Medicine, Department of Veterinary Medicine, University of Veterinary and Animal Sciences, Lahore, Pakistan.
Department of Veterinary Medicine, University of Veterinary and Animal Sciences, Lahore, Pakistan.
Iran J Vet Res. 2024;25(2):98-106. doi: 10.22099/IJVR.2024.48563.7095.
is a potential emerging and prevailing multidrug-resistant (MDR) pathogen involved in bovine endometritis.
Present research evaluated the prevalence and molecular characterization of methicillin-resistant (MRSA) and beta-lactam resistant (BRSA) and also analyzed the associated risk factors with endometritis along with antibiotic resistance patterns.
A total of 384 uterine and vaginal fluid samples were collected from the adult dairy cattle and buffaloes showing the clinical signs of endometritis including foul-smelling vaginal discharge, fever, enlarged and thickened uterine horns on rectal palpation, and confirmation by ultrasonography findings. The collected samples were subjected to standard microbiological methods for the detection of . The confirmed isolates were further subjected to the Kirby-Bauer disc diffusion test and the detection of the and genes for the confirmation of MRSA and BRSA.
Study found an overall prevalence of 17.96% for from bovine endometritis cases. Among isolates, 50.72% and 37.68% isolates were confirmed MRSA while BRSA was found as 36.23% and 18.84%, based on phenotypic and genotypic methods, respectively. Phylogenetic analysis indicated the possibility of pathogen transmission within and between livestock animals. Risk factor analysis showed that the breed of animal, visible discharge from vagina, lactation number, insemination procedure, and calving place showed significant (P<0.05) association with -associated endometritis. Antimicrobial susceptibility testing of study isolates showed the resistance to various commonly used antibiotics.
The study concluded that is found in 17.96% of bovines affected with endometritis and require further intensive research to elucidate the farm economic losses.
是一种潜在的新兴且普遍存在的耐多药（MDR）病原体，与牛子宫内膜炎有关。
本研究评估了耐甲氧西林金黄色葡萄球菌（MRSA）和耐β-内酰胺金黄色葡萄球菌（BRSA）的流行情况和分子特征，并分析了与子宫内膜炎相关的危险因素以及抗生素耐药模式。
从出现子宫内膜炎临床症状的成年奶牛和水牛中总共采集了384份子宫和阴道液体样本，这些症状包括阴道分泌物有异味、发热、直肠触诊时子宫角增大增厚，并通过超声检查结果进行确认。对采集的样本采用标准微生物学方法检测。对确诊的分离株进一步进行 Kirby-Bauer 纸片扩散试验以及检测 mecA 和 blaZ 基因以确认 MRSA 和 BRSA。
研究发现牛子宫内膜炎病例中该菌的总体患病率为17.96%。在分离株中，基于表型和基因型方法，分别有50.72%和37.68%的分离株被确认为MRSA，而BRSA分别为36.23%和18.84%。系统发育分析表明病原体在牲畜动物内部和之间传播的可能性。危险因素分析表明动物品种、阴道可见分泌物、泌乳次数、授精程序和产犊地点与该菌相关的子宫内膜炎有显著（P<0.05）关联。对研究分离株的抗菌药敏试验表明对各种常用抗生素有耐药性。
该研究得出结论，在17.96%的患子宫内膜炎的牛中发现了该菌，需要进一步深入研究以阐明农场的经济损失。
