Chen Lin, Tang Zi-Yun, Cui Shi-Yun, Ma Zhen-Bao, Deng Hua, Kong Wei-Li, Yang Li-Wen, Lin Chao, Xiong Wen-Guang, Zeng Zhen-Ling
Guangdong Provincial Key Laboratory of Veterinary Drugs Development and Safety Evaluation, National Risk Assessment Laboratory for Antimicrobial Resistance of Animal Original Bacteria, South China Agricultural University, Guangzhou 510642, China.
Guangdong Laboratory for Lingnan Modern Agriculture, South China Agricultural University, Guangzhou 510642, China.
Pathogens. 2020 Apr 4;9(4):264. doi: 10.3390/pathogens9040264.
() is one of the most clinically important zoonotic pathogens, but an understanding of the prevalence, biofilm formulation ability, virulence, and antimicrobial resistance genes of from veterinary hospitals is lacking. By characterizing in different origins of veterinary hospitals in Guangzhou, China, in 2019, we identified with the presence of in pets (17.1%), veterinarians (31.7%), airborne dust (19.1%), environmental surfaces (4.3%), and medical device surfaces (10.8%). Multilocus sequence typing (MLST) and protein A () typing analyses demonstrated methicillin-sensitive (MSSA) ST398-t571, MSSA ST188-t189, and methicillin-resistant (MRSA) ST59-t437 were the most prevalent lineage. with similar pulsed-field gel electrophoresis (PFGE) types distributed widely in different kinds of samples. The crystal violet straining assays revealed 100% (3/3) of MRSA ST59 and 81.8% (9/11) of MSSA ST188 showed strong biofilm formulation ability, whereas other STs (ST1, ST5, ST7, ST15, ST88, ST398, ST3154 and ST5353) showed weak biofilm production ability. Polymerase chain reaction (PCR) confirmed the most prevalent leucocidin, staphylococcal enterotoxins, operon, and adhesion genes were - (49.0%), - (15.7%), --- (100.0%), and ---- (100.0%), respectively. Our study showed that the isolates with strong biofilm production ability had a higher prevalence in , , and genes compared to the isolates with weak biofilm production ability. Furthermore, 2 ST1-MRSA isolates with gene and 1 ST88-MSSA isolate with gene were detected. In conclusion, the clonal dissemination of of different origins in veterinary hospitals may have occurred; the biofilm production capacity of is strongly correlated with ST types; some adhesion genes such as , , may pose an influence on biofilm production ability and the emergence of and genes in from veterinary hospitals should raise our vigilance.
（）是临床上最重要的人畜共患病原体之一，但目前缺乏对来自兽医医院的（）的流行情况、生物膜形成能力、毒力和抗菌药物耐药基因的了解。通过对2019年中国广州不同来源的兽医医院中的（）进行特征分析，我们确定在宠物（17.1%）、兽医（31.7%）、空气尘埃（19.1%）、环境表面（4.3%）和医疗器械表面（10.8%）中存在（）。多位点序列分型（MLST）和蛋白A（）分型分析表明，甲氧西林敏感（MSSA）ST398-t571、MSSA ST188-t189和耐甲氧西林（MRSA）ST59-t437是最常见的谱系。具有相似脉冲场凝胶电泳（PFGE）类型的（）广泛分布于不同类型的样本中。结晶紫染色试验显示，100%（3/3）的MRSA ST59和81.8%（9/11）的MSSA ST188具有很强的生物膜形成能力，而其他ST型（ST1、ST5、ST7、ST15、ST88、ST398、ST3154和ST5353）显示出较弱的生物膜产生能力。聚合酶链反应（PCR）证实，最常见的杀白细胞素、葡萄球菌肠毒素、（）操纵子和黏附基因分别为-（49.0%）、-（15.7%）、---（100.0%）和----（100.0%）。我们的研究表明，与生物膜产生能力较弱的分离株相比，具有较强生物膜产生能力的分离株在（）、（）、（）和（）基因中的流行率更高。此外，检测到2株携带（）基因的ST1-MRSA分离株和1株携带（）基因的ST88-MSSA分离株。总之，兽医医院中不同来源的（）可能已发生克隆传播；（）的生物膜产生能力与ST型密切相关；一些黏附基因如（）、（）、（）可能对生物膜产生能力有影响，兽医医院（）中（）和（）基因的出现应引起我们的警惕。
