Department of Clinical Medicine and Surgery University of Veterinary and Animal Sciences, Lahore, Pakistan.
J Parasitol. 2021 Mar 1;107(2):295-303. doi: 10.1645/20-50.
Anaplasmosis is a widespread vector-borne disease affecting dogs, and Anaplasma platys is the major etiological agent of the disease. The study examines anaplasmosis molecular prevalence, related risk factors, and alteration of hematological variables in Anaplasma-affected dogs. A total of 150 blood samples were collected from dogs in the district of Lahore, Pakistan. The samples were screened with PCR targeting the 16S rRNA gene of Anaplasma. Sequencing of samples that were found positive after performing PCR was conducted. A questionnaire was developed to collect epidemiological data on subject dogs, and the information was analyzed with a logistic regression model using SPSS. The current study revealed an 11.34% (17/150) prevalence of anaplasmosis in dogs based on PCR detection. Tick infestation, previous tick history, house hygiene, and tick control status were major risk factors linked with disease occurrence. Red blood cell count, packed cell volume, hemoglobin, and platelet count were decreased significantly (P < 0.05) in Anaplasma-infected dogs. Phylogenetically, the 2 isolates of the current study clustered together, and that cluster was very similar to A. platys isolates from India, Malaysia, and Thailand.
无形体病是一种广泛流行的犬类传染病，由无形体属的主要病原体——平壁无形体引起。本研究旨在调查无形体病在巴基斯坦拉合尔地区的分子流行率、相关危险因素以及对感染犬血液学参数的影响。研究共采集了 150 份来自拉合尔地区的犬血样。采用针对 16S rRNA 基因的 PCR 技术对样本进行检测，对 PCR 阳性样本进行测序。此外，还设计了一份问卷来收集有关研究犬的流行病学数据，并使用 SPSS 软件的逻辑回归模型对这些信息进行了分析。研究结果显示，基于 PCR 检测，犬无形体病的流行率为 11.34%（17/150）。犬只的蜱虫感染、既往蜱虫史、家庭卫生和蜱虫控制状况是与疾病发生相关的主要危险因素。感染犬的红细胞计数、红细胞压积、血红蛋白和血小板计数均显著降低（P<0.05）。从系统发育学角度来看，本研究中的 2 个分离株与来自印度、马来西亚和泰国的平壁无形体分离株聚为一簇，且相似度很高。
