Malik Javaria, Moosa Anam, Zulfiqar Faisal, Althobaiti Fayez, Andaleeb Hina, Alzamel Nurah M, Alsharari Sultan F, Fayad Eman, Al-Asmari Fahad, Şimşek Özhan, İzgü Tolga, Tütüncü Mehmet, Al-Duais Mohammed Ali, Ghobashy Madeha O I, Alwabsi Hayam A, Zidan Nahla, Sakran Mohamed, Soliman Tarek M A
Department of Plant Pathology, Faculty of Agriculture and Environment, The Islamia University of Bahawalpur, Bahawalpur, 63100, Pakistan.
Department of Horticultural Sciences, Faculty of Agriculture and Environment, The Islamia University of Bahawalpur, Bahawalpur, 63100, Pakistan.
Curr Microbiol. 2024 Dec 3;82(1):28. doi: 10.1007/s00284-024-03987-0.
Application of melatonin and lipopeptides (LPs) derived from Bacillus strains is considered an efficient strategy to control plant diseases at both pre and postharvest stages. However, the combined application of melatonin and LPs has not been studied yet. Therefore, the present study presents the synergistic effect of melatonin and LPs produced by Bacillus atrophaeus strain MCM61 against gray mold disease and its impact on quality parameters and vase life of cut roses. The stems of cut roses along with flowers were dipped in the melatonin solution at concentration (0, 0.2, 0.4, 0.6, and 0.8 mM) and the results indicated that the vase life of flowers treated with the melatonin was enhanced compared to control. Melatonin at 0.6 mM concentration showed the highest vase life of cut roses at day 8. Synergistic treatment with melatonin and LPs of B. atrophaeus MCM61 revealed that MDA and HO content showed the highest decrease in cut roses. Similarly, relative water content, total phenol content, GSH content, and defense enzymes i.e., APX, SOD, PPO, POD, and CAT activities were increased in cut roses treated with co-application of B. atrophaeus MCM61 LPs and melatonin compared to single treatments and control treatment. Furthermore, the longevity of cut roses was also improved in flowers treated with a combined application of MCM61 and melatonin compared to other treatments.
褪黑素和源自芽孢杆菌菌株的脂肽（LPs）的应用被认为是在收获前和收获后阶段控制植物病害的有效策略。然而，褪黑素和LPs的联合应用尚未得到研究。因此，本研究展示了萎缩芽孢杆菌菌株MCM61产生的褪黑素和LPs对灰霉病的协同作用及其对切花玫瑰品质参数和瓶插寿命的影响。将切花玫瑰的茎连同花朵浸入浓度为（0、0.2、0.4、0.6和0.8 mM）的褪黑素溶液中，结果表明，与对照相比，用褪黑素处理的花朵的瓶插寿命有所延长。浓度为0.6 mM的褪黑素在第8天显示出切花玫瑰的最高瓶插寿命。褪黑素与萎缩芽孢杆菌MCM61的LPs的协同处理表明，切花玫瑰中的丙二醛（MDA）和过氧化氢（HO）含量下降最多。同样，与单一处理和对照处理相比，联合应用萎缩芽孢杆菌MCM61的LPs和褪黑素处理的切花玫瑰的相对含水量、总酚含量、谷胱甘肽（GSH）含量以及防御酶即抗坏血酸过氧化物酶（APX）、超氧化物歧化酶（SOD）、多酚氧化酶（PPO）、过氧化物酶（POD）和过氧化氢酶（CAT）的活性均有所增加。此外，与其他处理相比，联合应用MCM61和褪黑素处理的花朵中切花玫瑰的寿命也得到了提高。
