Zhang Shengcan, He Xinlin, Liu Fang, Huang Xiangya, Mai Sui, He Jingwei
College of Materials Science and Engineering, South China University of Technology, Guangzhou, China.
Hospital of Stomatology, Sun Yat-sen University, Guangzhou, China; Guangdong Provincial Key Laboratory of Stomatology, Guangzhou, China; Institute of Stomatology, Sun Yat-sen University, Guangzhou, China.
Dent Mater. 2025 Feb;41(2):169-178. doi: 10.1016/j.dental.2024.11.010. Epub 2024 Dec 2.
The purpose of this study was to enhance the anti-bacterial adhesion effect against Streptococcus mutans (S. mutans) of fluorinated dimethacrylate (DF MA) based dental resin composites (DRCs) by using silicone dimethacrylate (SMA-MEO).
The SMA-MEO was added into mixture of DFMA and tricyclo (5.2.1.0) decanedimethanol diacrylate (SR833s) (DFMA/SR833s = 50 wt./50 wt.) with mass ratios of 10 wt% and 20 wt% to form resin matrix both with fluorinated and silicon containing dimethacrylates, and then DRCs named DS+ 10 %SMA-MEO and DS+ 20 %SMA-MEO were prepared by mixing the resin matrix with silaned BaAlSiO filler particles at a mass ratio of 30 wt./70 wt. Double bond conversion, volumetric shrinkage and shrinkage stress, flexural strength and modulus, water sorption and solubility, contact angle and surface free energy, anti-bacterial adhesion effect against Streptococcus mutans (S. mutans), and cytotoxicity of prepared DRCs were investigated according to standard or referenced methods. Fluorinated dimethacrylate (DFMA) based DRC named DS and 2,2-bis[4-(2-hydroxy-3-methacryloy-loxypropyl)-phenyl]propane (Bis-GMA) based DRC named BT were used as controls.
Adding SMA-MEO into DFMA based DRC could lead to higher double bond conversion (p < 0.05), higher hydrophobicity (p < 0.05), and lower surface free energy (p < 0.05). Only DS+ 10 %SMA-MEO had better anti-bacterial adhesion effect against S. mutans than DS (p < 0.05). The SMA-MEO had no influence on volumetric shrinkage, shrinkage stress, flexural modulus, water sorption and solubility of DRC (p > 0.05), but could reduce flexural strength of dry DRC (p < 0.05). After water immersion, SMA-MEO containing DRCs had comparable flexural strength as DS (p > 0.05). Compared with BT, DS and SMA-MEO containing DRCs had better or comparable physicochemical properties, and lower amount of adherent S. mutans. All of DRCs had comparable cytotoxicity (p > 0.05).
DRCs with both DFMA and SMA-MEO could have better anti-bacterial adhesion effect against S. mutans than DRC only with DFMA due to increased hydrophobicity and decreased Surface free energy, and the optimal mass fraction of SMA-MEO in DFMA based resin matrix was 10 wt%.
本研究旨在通过使用二甲基硅氧烷甲基丙烯酸酯（SMA-MEO）来增强含氟二甲基丙烯酸酯（DF MA）基牙科树脂复合材料（DRCs）对变形链球菌（S. mutans）的抗菌黏附效果。
将SMA-MEO以10 wt%和20 wt%的质量比添加到DFMA与三环（5.2.1.0）癸烷二甲醇二丙烯酸酯（SR833s）（DFMA/SR833s = 50 wt./50 wt.）的混合物中，形成含氟和含硅二甲基丙烯酸酯的树脂基体，然后将该树脂基体与硅烷化的BaAlSiO填料颗粒按30 wt./70 wt.的质量比混合，制备出名为DS + 10%SMA-MEO和DS + 20%SMA-MEO的DRCs。根据标准或参考方法，对制备的DRCs的双键转化率、体积收缩率和收缩应力、弯曲强度和模量、吸水性和溶解性、接触角和表面自由能、对变形链球菌（S. mutans）的抗菌黏附效果以及细胞毒性进行了研究。以含氟二甲基丙烯酸酯（DFMA）基的DRC名为DS和以2,2-双[4-(2-羟基-3-甲基丙烯酰氧基丙基)-苯基]丙烷（Bis-GMA）基的DRC名为BT作为对照。
向基于DFMA的DRC中添加SMA-MEO可导致更高的双键转化率（p < 0.05）、更高的疏水性（p < 0.05）和更低的表面自由能（p < 0.05）。只有DS + 10%SMA-MEO对变形链球菌的抗菌黏附效果比DS更好（p < 0.05）。SMA-MEO对DRC的体积收缩率、收缩应力、弯曲模量、吸水性和溶解性没有影响（p > 0.05），但会降低干燥DRC的弯曲强度（p < 0.05）。水浸泡后，含SMA-MEO的DRCs的弯曲强度与DS相当（p > 0.05）。与BT相比，含DS和SMA-MEO的DRCs具有更好或相当的物理化学性质，且变形链球菌的黏附量更低。所有DRCs的细胞毒性相当（p > 0.05）。
由于疏水性增加和表面自由能降低，同时含有DFMA和SMA-MEO的DRCs对变形链球菌的抗菌黏附效果比仅含DFMA的DRCs更好，且基于DFMA的树脂基体中SMA-MEO的最佳质量分数为10 wt%。
