Kuryntseva Polina Aleksandrovna, Pronovich Nataliya Andreevna, Bukarinova Yulia Olegovna, Khlebova Darya Lvovna, Kamalova Alina Rinatovna, Khamieva Aliia Nailevna, Khamiev Marsel Maratovich, Galitskaya Polina Yurevna, Selivanovskaya Svetlana Yurevna
Institute of Ecology, Biotechnology and Nature Management, Kazan (Volga Region) Federal University, Kazan, 420008, Russia.
Institute of Geology and Petroleum Technologies, Kazan (Volga Region) Federal University, Kazan, 420111, Russia.
World J Microbiol Biotechnol. 2024 Dec 4;40(12):404. doi: 10.1007/s11274-024-04207-4.
Chelating agents used in agriculture and land reclamation practices must exhibit the capacity to firmly bind metal ions while also rapidly decomposing into non-toxic compounds due to their introduction into the environment in substantial quantities. It is therefore crucial to identify an alternative to EDTA, a prevalent chelating agent known for its low biodegradability and relatively high toxicity. This study focuses on assessing the degree of biodegradation of iminoglutamic succinic acid (IGSA) and the toxicity of its half-decomposition products. For this purpose, two bacterial and fungal isolates capable of decomposing IGSA were isolated and characterized, identified as Ralstonia pickettii and Fusarium foetens, respectively. The results of a 28-day experiment demonstrated that Ralstonia pickettii was capable of decomposing IGSA by 18.0% according to the manometric test and by 24.5% based on the analysis of decomposition products via HPLC. In comparison, Fusarium foetens exhibited a more effective biodegradation capacity, with rates of 20.3% and 32.5%, respectively. The half-decomposition products of IGSA were characterized by low ecotoxicity levels (LID10) concerning Paramecium caudatum (11.2-13.2 g/l) and Ceriodaphnia affinis (3.6-8.9 g/l). However, a stimulating effect was observed on microalgae, with growth stimulation ranging from 63.65-96.60%.
农业和土地复垦实践中使用的螯合剂必须具备牢固结合金属离子的能力，同时由于大量引入环境中，还需能迅速分解为无毒化合物。因此，找到一种替代乙二胺四乙酸（EDTA）的物质至关重要，EDTA是一种常见的螯合剂，以其低生物降解性和相对较高的毒性而闻名。本研究聚焦于评估亚氨基谷氨酸琥珀酸（IGSA）的生物降解程度及其半分解产物的毒性。为此，分离并鉴定了两种能够分解IGSA的细菌和真菌菌株，分别鉴定为皮氏罗尔斯通氏菌和恶臭镰刀菌。一项为期28天的实验结果表明，根据压力测试，皮氏罗尔斯通氏菌能够分解18.0%的IGSA，通过高效液相色谱法分析分解产物得出的分解率为24.5%。相比之下，恶臭镰刀菌表现出更有效的生物降解能力，分解率分别为20.3%和32.5%。IGSA的半分解产物对尾草履虫（11.2 - 13.2微克/升）和近缘哲水蚤（3.6 - 8.9微克/升）的生态毒性水平较低（LID10）。然而，观察到对微藻有刺激作用，生长刺激范围为63.65 - 96.60%。
