Guo Yingjian, Yu Jinqian, Wang Chuangchuang, Wang Zhenqiang, Niu Tong, Li Jia, Wang Xiao
School of Pharmaceutical Sciences, Shandong University of Traditional Chinese Medicine, Jinan, China.
Key Laboratory for Natural Active Pharmaceutical Constituents Research in Universities of Shandong Province, School of Pharmaceutical Sciences, Qilu University of Technology (Shandong Academy of Sciences), Jinan, China.
Nat Prod Res. 2024 Dec 3:1-7. doi: 10.1080/14786419.2024.2431999.
The total iridoid glycosides (TIG) from Thunb. were firstly enriched by D316 anion exchange resin, using the response surface method (RSM) model to optimise the enrichment parameters, leading to the optimised RSM parameters of 1.25 g sample weight, 8.83 h adsorption time, 3.65 BV elution volume. Quantitative analysis of TIG showed the content increased from 12.71 mg/g to 74.62 mg/g after enrichment. The TIG obtained was analysed by UPLC-Q-TOF-MS/MS, identifying 34 iridoid glycosides. The anti-inflammatory activity of TIG was evaluated by LPS-induced RAW264.7 cells , effectively inhibiting the production of NO at the concentrations of 0.8,1.6, 3.2 mg/mL. TIG from were successfully enriched by D316 resin to remove the abundant phenolic acids through acid-base reaction, which provided material basis for the further efficacious constituents revelation for , as well as the further exploitation of TIG as anti-inflammatory drug.
首次采用D316阴离子交换树脂对Thunb.中的总环烯醚萜苷（TIG）进行富集，利用响应面法（RSM）模型优化富集参数，得到优化后的RSM参数为样品重量1.25 g、吸附时间8.83 h、洗脱体积3.65 BV。TIG的定量分析表明，富集后其含量从12.71 mg/g增加到74.62 mg/g。对所得TIG进行UPLC-Q-TOF-MS/MS分析，鉴定出34种环烯醚萜苷。通过脂多糖诱导的RAW264.7细胞评价TIG的抗炎活性，在0.8、1.6、3.2 mg/mL浓度下能有效抑制NO的产生。通过D316树脂成功富集了Thunb.中的TIG，通过酸碱反应去除了大量酚酸，为进一步揭示Thunb.的有效成分以及将TIG开发为抗炎药物提供了物质基础。
