Zhao Linlin, Yin Guobin, Zhang Yonglin, Duan Chaofan, Wang Yang, Kang Zhen
The Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China.
The Science Center for Future Foods, Jiangnan University, Wuxi 214122, China.
Eng Microbiol. 2022 Feb 21;2(1):100012. doi: 10.1016/j.engmic.2022.100012. eCollection 2022 Mar.
is the most well-studied model prokaryote and has become an indispensable host for the biotechnological production of proteins and biochemicals. In particular, the probiotic status of one Nissle 1917 (EcN) has helped it become a new favorite amongst synthetic biologists. To broaden its potential applications, here we assemble a comparative study on the genomes, transcriptomes, and metabolic properties of strains EcN, BL21(DE3), and MG1655. Comparative genomics data suggests that EcN possesses 1404 unique CDSs. In particular, EcN has additional iron transport systems which endow EcN with a higher tolerance to iron scarcity when compared to two other strains. EcN transcriptome data demonstrates that strains EcN, BL21(DE3), and MG1655 all have comparable activities of the central metabolic pathway, however only EcN inherits the arginine deiminase pathway. Additionally, we found that EcN displayed a lower expression of ribosomal proteins compared to BL21(DE3) and MG1655. This comparative study on strains EcN, BL21(DE3), and MG1655 aims to provide a reference for further engineering EcN as a biotechnological tool.
是研究最充分的模式原核生物，已成为蛋白质和生物化学品生物技术生产中不可或缺的宿主。特别是，一株1917年的Nissle大肠杆菌（EcN）的益生菌特性使其成为合成生物学家的新宠。为了拓宽其潜在应用，我们在此对EcN菌株、BL21(DE3)菌株和MG1655菌株的基因组、转录组和代谢特性进行了比较研究。比较基因组学数据表明，EcN拥有1404个独特的编码序列。特别是，EcN具有额外的铁转运系统，与其他两种菌株相比，这使EcN对铁缺乏具有更高的耐受性。EcN转录组数据表明，EcN菌株、BL21(DE3)菌株和MG1655菌株的中心代谢途径活性相当，但只有EcN继承了精氨酸脱亚胺酶途径。此外，我们发现与BL21(DE3)和MG1655相比，EcN的核糖体蛋白表达较低。对EcN菌株、BL21(DE3)菌株和MG1655菌株的这项比较研究旨在为进一步将EcN工程化为生物技术工具提供参考。
