Farda Beatrice, Djebaili Rihab, Sabbi Enrico, Pagnani Giancarlo, Cacchio Paola, Pellegrini Marika
Department of Life, Health and Environmental Sciences, University of L'Aquila, 67100 L'Aquila, Italy.
Department of Bioscience and Technologies for Food, Agriculture and Environment, University of Teramo, Teramo, 64100, Italy.
AIMS Microbiol. 2024 Nov 8;10(4):944-972. doi: 10.3934/microbiol.2024041. eCollection 2024.
Sustainable alternatives are essential to improving agriculture production to meet the growing world's critical demands. Cyanobacteria and microalgae are considered renewable resources with a wide range of potential uses in the agricultural sector. We aimed to isolate cyanobacteria and microalgae from the mud of a carbon dioxide-rich sulfur pond and to investigate their plant growth-promoting (PGP) and soil bio-consolidating ability. Mud samples were subjected to DNA extraction and 16S rRNA gene sequencing to characterize the prokaryotic community. Phototrophic culturable microbiota was isolated and evaluated for different PGP properties. The most relevant isolates were combined in a consortium and used for bioconsolidation activity. In a greenhouse experiment, the isolates were evaluated for their ability to promote salinity stress tolerance in sunflower plants. Metabarcoding results showed that most Amplicon Sequence Variants (ASV) were associated with Actinobacteriota (35%), Proteobacteria (19%), and Acidobacteriota (11%) at the phylum level and (32%) and uncultured (31%) lineages at the genus level. The culture-dependent method yielded eight isolates associated with cyanobacteria and microalgae genera. The isolates obtained showed interesting PGP activities. Isolates C1, C2, and M1 were selected based on phosphate solubilization (85.6 µg PO mL on average), indoles (C1 and M1 0.54 µg mL IAA equivalents on average), and ACC deaminase activity (C2 and M1 6.00 µmol α-KB mg proteins h. The consortium efficiently consolidated sand particles in the presence of calcium carbonate by forming biomineralized aggregates. results showed positive effects of the consortium on ., plant growth under normal conditions and salt stress. The positive effects on soil and plants indicated their effectiveness as bioconsolidants and biostimulant agents. Our findings highlight the interesting potential of cyanobacteria and microalgae applications in sustainable agriculture.
可持续替代方案对于提高农业产量以满足全球日益增长的关键需求至关重要。蓝细菌和微藻被认为是可再生资源，在农业领域具有广泛的潜在用途。我们旨在从富含二氧化碳的硫磺池塘的泥浆中分离蓝细菌和微藻，并研究它们促进植物生长（PGP）和土壤生物固结的能力。对泥浆样品进行DNA提取和16S rRNA基因测序，以表征原核生物群落。分离出光合可培养微生物群，并评估其不同的PGP特性。将最相关的分离株组合成一个联合体，并用于生物固结活性研究。在温室实验中，评估这些分离株促进向日葵植物耐盐胁迫的能力。元条形码结果表明，在门水平上，大多数扩增子序列变体（ASV）与放线菌门（35%）、变形菌门（19%）和酸杆菌门（11%）相关，在属水平上与（32%）和未培养（31%）的谱系相关。基于培养的方法产生了8株与蓝细菌和微藻属相关的分离株。获得的分离株显示出有趣的PGP活性。根据平均85.6μg PO/mL的解磷能力、吲哚（C1和M1平均0.54μg/mL IAA当量）和ACC脱氨酶活性（C2和M1 6.00μmolα-KB mg蛋白质/h）选择分离株C1、C2和M1。该联合体在碳酸钙存在下通过形成生物矿化聚集体有效地固结了沙粒。结果表明该联合体对正常条件下和盐胁迫下的植物生长有积极影响。对土壤和植物的积极影响表明它们作为生物固结剂和生物刺激剂的有效性。我们的研究结果突出了蓝细菌和微藻在可持续农业中的有趣潜力。
