School of Biomedical Engineering, McMaster University, 1280 Main Street West, Hamilton, Ontario, L8S 4K1, Canada.
Department of Biochemistry and Biomedical Sciences, McMaster University, Canada.
Angew Chem Int Ed Engl. 2022 Aug 1;61(31):e202204252. doi: 10.1002/anie.202204252. Epub 2022 Jun 9.
Pen-side testing of farm animals for infectious diseases is critical for preventing transmission in herds and providing timely intervention. However, most existing pathogen tests have to be conducted in centralized labs with sample-to-result times of 2-4 days. Herein we introduce a test that uses a dual-electrode electrochemical chip (DEE-Chip) and a barcode-releasing electroactive aptamer for rapid on-farm detection of porcine epidemic diarrhea viruses (PEDv). The sensor exploits inter-electrode spacing reduction and active field mediated transport to accelerate barcode movement from electroactive aptamers to the detection electrode, thus expediting assay operation. The test yielded a clinically relevant limit-of-detection of 6 nM (0.37 μg mL ) in saliva-spiked PEDv samples. Clinical evaluation of this biosensor with 12 porcine saliva samples demonstrated a diagnostic sensitivity of 83 % and specificity of 100 % with a concordance value of 92 % at an analysis time of one hour.
对农场动物进行传染性疾病的笔式检测对于预防畜群传播和及时干预至关重要。然而，大多数现有的病原体检测都必须在集中的实验室中进行，样本到结果的时间为 2-4 天。在此，我们介绍了一种使用双电极电化学芯片（DEE-Chip）和释放条码的电化学生物适体进行快速农场检测猪流行性腹泻病毒（PEDv）的方法。该传感器利用电极间距离的缩小和活性场介导的输运来加速条码从电化学生物适体向检测电极的运动，从而加速检测操作。该测试在唾液中添加 PEDv 样本时，在临床上具有 6 nM（0.37 μg/mL）的相关检测限。用 12 个猪唾液样本对这种生物传感器进行临床评估，在 1 小时的分析时间内，其诊断灵敏度为 83%，特异性为 100%，一致性值为 92%。
