Genomic epidemiology and genetic characteristics of clinical species cocirculating in West Bengal, India, 2019, using whole genome analysis.

species are the most common pathogens responsible for foodborne gastroenteritis worldwide. India is a region with frequent diarrheal infections and a high level of infection incidence, but the detailed genomic information is limited. This study aimed to characterize 112 isolates of from diarrhea patients at two hospitals in Kolkata, West Bengal, by whole genome analysis. The isolates consisted of 90 . , 20 . , and 2 . isolates. Multilocus sequence typing analysis revealed that the largest sequence type (ST) populations were ST-2131 in and ST-830 in and seven novel STs were found in and one in . Notably, ST-2131, which is rarely seen elsewhere, was positive for a sialylated LOS-related gene () associated with Guillain-Barré syndrome. Antibiotic resistance factors predicted from the genome sequence included variants (58.9%), (54.5%), (0.9%), (0.9%), mutation in GyrA (T86I, T86I+D90N, T86I+P104S, T86I+D90+P104S) (79.5%), and mutation in 23S rRNA (A2075G) (12.5%). In addition to the high drug resistance of in Kolkata, pathogens were circulating that may be associated with Guillain-Barré syndrome. This study indicates the importance of genomic analysis in the surveillance of pathogens, which provides genomic information on genetic diversity, virulence mechanisms, and determinants of antimicrobial resistance.