Balestrino F, Puggioli A, Mamai W, Bellini R, Bouyer J
Insect Pest Control Laboratory IPCL, Joint FAO/IAEA Centre of Nuclear Techniques in Food and Agriculture, IAEA, Seibersdorf, 2444, Austria.
Sanitary Entomology and Zoology Department, Centro Agricoltura Ambiente ″G. Nicoli″, Crevalcore, 40014, Italy.
Sci Rep. 2024 Dec 4;14(1):30276. doi: 10.1038/s41598-024-81385-z.
The sterile insect technique (SIT) is a sustainable genetic control method to effectively suppress mosquito vector populations below sanitary and nuisance thresholds. The SIT requires a cost-effective production and release of radio sterilized insects of high and consistent quality and quantity. In this study, we report a new procedure to hatch, aliquot and introduce Aedes albopictus and Aedes aegypti larvae into larval mass rearing units using dissolvable vegetable capsules containing brushed eggs and larval diet. The results demonstrated that the egg brushing procedures, the opening of the hatching container and the presence of diet in the hatching solution increased Aedes eggs hatching success. The diet added during the hatching process decreased the dissolved oxygen (DO) concentration, which constituted the main stimulus for egg hatching success in both species. However, a DO reduction below 3 mg/l can effectively stimulate hatching in both species regardless of the deoxygenation method adopted. The capsule method produced similar hatching success without affecting larval survival and pupation rates in comparison with standard hatching procedures. The use of capsules eliminates intermediate laborious procedures for hatching, counting and transferring larvae into mass rearing trays with an expected improvement in production times and costs for Aedes SIT operational programs.
昆虫不育技术（SIT）是一种可持续的遗传控制方法，可有效将蚊媒种群抑制在卫生和滋扰阈值以下。昆虫不育技术需要以具有成本效益的方式生产和释放高质量、数量稳定且经过辐射绝育的昆虫。在本研究中，我们报告了一种新方法，即使用装有刷下的卵和幼虫饲料的可溶解蔬菜胶囊，将白纹伊蚊和埃及伊蚊幼虫孵化、分装并引入幼虫大规模饲养单元。结果表明，刷卵程序、孵化容器的打开以及孵化溶液中饲料的存在提高了伊蚊卵的孵化成功率。孵化过程中添加的饲料降低了溶解氧（DO）浓度，这是两种伊蚊卵孵化成功的主要刺激因素。然而，无论采用何种脱氧方法，DO浓度降至3毫克/升以下均可有效刺激两种伊蚊的孵化。与标准孵化程序相比，胶囊法产生了相似的孵化成功率，且不影响幼虫存活率和化蛹率。使用胶囊消除了孵化、计数以及将幼虫转移到大规模饲养托盘等中间繁琐程序，有望缩短埃及伊蚊昆虫不育技术操作项目的生产时间并降低成本。
