Insect Pest Control Laboratory, Joint FAO/IAEA Centre of Nuclear Techniques in Food and Agriculture, Vienna, Austria.
Institut de Recherche Agricole pour le Développement (IRAD), Yaoundé, Cameroun.
Sci Rep. 2024 Apr 22;14(1):9170. doi: 10.1038/s41598-024-58067-x.
This study provides a comparative analysis of two state-of-the-art automatic mosquito pupae sex sorters currently available: the ORINNO and the WOLBAKI Biotech pupae sex separation systems, which both exploit the sexual size dimorphism of pupae. In Aedes aegypti, the WOLBAKI sex sorter and the ORINNO with a sieve mesh size of 1.050 mm achieved sex separation with female contamination rates below 1%, low pupae mortality rates and high male flight capacity. However, in Ae. albopictus, there was more variability, with female contamination rates above the 1% threshold and pupae mortality reaching 27% when using the ORINNO sorter. On the other hand, the WOLBAKI sorter achieved a male pupae recovery of 47.99 ± 8.81% and 50.91 ± 11.77% in Ae. aegypti and Ae. albopictus, respectively, while the ORINNO sorter with a smaller sieve size achieved male pupae recoveries of 38.08 ± 9.69% and 40.16 ± 2.73% in Ae. aegypti and Ae. albopictus, respectively. This study provides valuable information for researchers and practitioners in the field, assisting in the selection of the most suitable system for mosquito control, management and research programs depending on their specific requirements.
ORINNO 和 WOLBAKI 生物技术蚊虫蛹性别分离系统，它们都利用了蛹的性大小二态性。在埃及伊蚊中，WOLBAKI 性别分拣器和 ORINNO（筛网尺寸为 1.050 毫米）的性别分离率低于 1%，雌性污染率低，雄性飞行能力高。然而，在白纹伊蚊中，情况更加复杂，使用 ORINNO 分拣器时，雌性污染率超过 1%的阈值，死亡率达到 27%。另一方面，WOLBAKI 分拣器在埃及伊蚊和白纹伊蚊中的雄性蛹回收率分别为 47.99±8.81%和 50.91±11.77%，而筛网尺寸较小的 ORINNO 分拣器在埃及伊蚊和白纹伊蚊中的雄性蛹回收率分别为 38.08±9.69%和 40.16±2.73%。本研究为该领域的研究人员和从业者提供了有价值的信息，根据他们的具体要求，协助选择最适合蚊虫控制、管理和研究计划的系统。
