Maïga Hamidou, Lu Deng, Mamai Wadaka, Bimbilé Somda Nanwintoum Séverin, Wallner Thomas, Bakhoum Mame Thierno, Bueno Masso Odet, Martina Claudia, Kotla Simran Singh, Yamada Hanano, Salvador Herranz Gustavo, Argiles Herrero Rafael, Chong Chee Seng, Tan Cheong Huat, Bouyer Jeremy
Insect Pest Control Laboratory, Joint FAO/IAEA Centre of Nuclear Techniques in Food and Agriculture, Department of Nuclear Sciences and Applications, IAEA Laboratories, Seibersdorf, Austria.
Institut de Recherche en Sciences de la Santé/Direction Régionale de l'Ouest (IRSS-DRO), Bobo-Dioulasso, Burkina Faso.
Front Bioeng Biotechnol. 2022 Jul 18;10:876675. doi: 10.3389/fbioe.2022.876675. eCollection 2022.
Successful implementation of the sterile insect technique (SIT) against relies on maintaining a consistent release of high-quality sterile males. Affordable, rapid, practical quality control tools based on the male's flight ability (ability to escape from a flight device) may contribute to meeting this requirement. Therefore, this study aims to standardize the use of the original FAO/IAEA rapid quality control flight test device (FTD) (version 1.0), while improving handling conditions and reducing the device's overall cost by assessing factors that could impact the subsequent flight ability of mosquitoes. The new FTD (version 1.1) is easier to use. The most important factors affecting escape rates were found to be tube color (or "shade"), the combined use of a lure and fan, mosquito species, and mosquito age and density (25; 50; 75; 100 males). Other factors measured but found to be less important were the duration of the test (30, 60, 90, 120 min), fan speed (normal 3000 rpm vs. high 6000 rpm), and mosquito strain origin. In addition, a cheaper version of the FTD (version 2.0) that holds eight individual tubes instead of 40 was designed and successfully validated against the new FTD (version 1.1). It was sensitive enough to distinguish between the effects of cold stress and high irradiation dose. Therefore, the eight-tube FTD may be used to assess flight ability. This study demonstrated that the new designs (versions 1.1 and 2.0) of the FTD could be used for standard routine quality assessments of mosquitoes required for an SIT and other male release-based programs.
成功实施针对[蚊虫名称未给出]的昆虫不育技术（SIT）依赖于持续释放高质量的不育雄虫。基于雄虫飞行能力（从飞行装置逃脱的能力）的经济、快速、实用的质量控制工具可能有助于满足这一要求。因此，本研究旨在规范粮农组织/国际原子能机构原始快速质量控制飞行测试装置（FTD）（版本1.0）的使用，同时通过评估可能影响[蚊虫名称未给出]后续飞行能力的因素来改善操作条件并降低装置的总体成本。新的FTD（版本1.1）更易于使用。发现影响逃脱率的最重要因素是管子颜色（或“色调”）、诱捕器和风扇的组合使用、蚊虫种类以及蚊虫年龄和密度（25只；50只；75只；100只雄虫）。其他测量但发现不太重要的因素是测试持续时间（30、60、90、120分钟）、风扇速度（正常3000转/分钟与高速6000转/分钟）以及蚊虫品系来源。此外，设计了一种更便宜的FTD版本（版本2.0），它可容纳八个单独的管子而非40个，并成功针对新的FTD（版本1.1）进行了验证。它足够灵敏，能够区分冷应激和高辐照剂量的影响。因此，八管FTD可用于评估[蚊虫名称未给出]的飞行能力。本研究表明，FTD的新设计（版本1.1和2.0）可用于昆虫不育技术及其他基于雄虫释放的项目所需的[蚊虫名称未给出]蚊虫的标准常规质量评估。
