Baudišová Dana, Bobková Šárka, Pumann Petr
National Institute of Public Health, Šrobárova 48/49, 100 42, Prague 10, Czech Republic.
Folia Microbiol (Praha). 2025 Feb;70(1):253-257. doi: 10.1007/s12223-024-01229-1. Epub 2024 Dec 5.
The aim of this study is to evaluate opportunistic pathogenic bacteria of the genus Pseudomonas in anthropogenically impacted bathing waters, primarily focusing on bathing ponds. The findings include the detection of these bacteria, their susceptibility to selected antibiotics, and the determination of the Exotoxin A (exoA) gene using PCR method. P. aeruginosa was present in most samples, albeit in low concentrations (1-14 CFU/100 mL). The presence of P. otitidis, which is associated with ear infection, in this type of bathing water, was not rare (up to 90 CFU/100 mL). This species would not be detected by the standard methods, including tests on acetamid medium, used for P. aeruginosa in water. The isolated strains of P. otitidis lack the exoA gene and exhibited higher resistance to meropenem compared to P. aeruginosa.
本研究的目的是评估受人为影响的浴场水中假单胞菌属的机会致病菌，主要聚焦于浴场池塘。研究结果包括这些细菌的检测、它们对所选抗生素的敏感性，以及使用聚合酶链式反应（PCR）方法测定外毒素A（exoA）基因。大多数样本中都存在铜绿假单胞菌，尽管其浓度较低（1 - 14 CFU/100 mL）。在这类浴场水中，与耳部感染相关的耳炎假单胞菌并不罕见（高达90 CFU/100 mL）。包括用于检测水中铜绿假单胞菌的乙酰胺培养基检测在内的标准方法无法检测到该菌种。分离出的耳炎假单胞菌菌株缺乏exoA基因，并且与铜绿假单胞菌相比，对美罗培南表现出更高的耐药性。
