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具有椭圆光束激发的高速小型化多光子显微镜

High Speed Miniaturized Multiphoton Microscopy with Elliptical Beam Excitation.

作者信息

Mattison Ben, Liu Shing-Jiuan, Tian Feng, Yang Weijian

机构信息

Department of Biomedical Engineering, University of California, Davis, Davis, CA 95616, USA.

Biomedical Engineering Graduate Group, University of California, Davis, Davis, CA 95616, USA.

出版信息

Biomed Opt. 2023 Apr;2023. doi: 10.1364/boda.2023.jtu4b.33.

Abstract

We demonstrate elliptical beam shaping in a miniaturized two-photon microscope enabling cellular resolution fluorescence imaging at over three times faster frame rate than point scanning over a 400 μm diameter FOV.

摘要

我们展示了在小型化双光子显微镜中的椭圆光束整形技术,该技术能够以比在直径400μm视场进行点扫描快三倍以上的帧率实现细胞分辨率荧光成像。

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Miniature three-photon microscopy maximized for scattered fluorescence collection.
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