Dang Shanshan, Mo Yanmei, Zeng Junqing, Xu Yunjie, Xie Zhongjian, Zhang Han, Zhang Bin, Nie Guohui
Shenzhen Key Laboratory of Nanozymes and Translational Cancer Research, Institute of Translational Medicine, Department of Otolaryngology, Shenzhen Second People's Hospital, the First Affiliated Hospital of Shenzhen University, Health Science Center, Institute of Microscale Optoelectronics, Shenzhen 518035, China,
Graduate Collaborative Training Base of Shenzhen Second' People's Hospital, Hengyang Medical School, University of South China, Hengyang, Hunan, 421001, China,
Nanophotonics. 2022 Aug 5;11(22):5061-5075. doi: 10.1515/nanoph-2022-0268. eCollection 2022 Dec.
As a key branch of the cross-discipline biophotonics, phototherapy, including photodynamic therapy (PDT), and photothermal therapy (PTT), is promising in biomedicine and visible light-driving PDT has been applied to clinical treatment. However, extensive applications of phototherapy are limited by the hypoxic microenvironment, laser penetration depth, and potential complexity for combined PDT/PTT. Thus, NIR-responsive oxygen self-supply nanocomposites functionalized with photosensitizers for achieving simultaneous in-depth PDT/PTT are urgently required. Herein, a multifunctional platform has been fabricated by co-immobilizing monodispersed ultrasmall Pd nanoclusters and a photosensitizer 5,10,15,20-Tetrakis (4-Aminophenyl)-21H,23H Porphyrin (Thp) on the surface of TiCT MXene nanosheets, generating the Pd-Thp-TiCT nanocomposite. Material characterization demonstrated that Pd nanoclusters and Thp were well-distributed on the MXene surface while MXene maintained its photothermal conversion efficiency and broad absorption. In this nanoplatform, irradiated by the single 808 nm laser, Pd selectively catalyzed the decomposition of HO to O, and O was continuously supplied to Thp for enhanced NIR-driving PDT. The fluorescence and photothermal imaging demonstrated the pronounced accumulation of nanocomposites in the tumor site. Both and results clearly demonstrated the nanocomposite had good biocompatibility, and that the synergistic PTT and enhanced PDT made apoptosis of the tumor cell achievable. This work not only proves this Pd-Thp-TiCT nanocomposite serves a promising solution for tumor hypoxia by inducing apoptosis of tumor cells with synergistic PTT and PDT, but also broadens the application of promising optical materials in biomedical field.
作为交叉学科生物光子学的一个关键分支，光疗法，包括光动力疗法（PDT）和光热疗法（PTT），在生物医学领域具有广阔前景，且可见光驱动的PDT已应用于临床治疗。然而，光疗法的广泛应用受到缺氧微环境、激光穿透深度以及PDT/PTT联合应用潜在复杂性的限制。因此，迫切需要用于实现同步深度PDT/PTT的近红外响应性氧自供应纳米复合材料，该材料由光敏剂功能化。在此，通过将单分散超小钯纳米簇和光敏剂5,10,15,20-四（4-氨基苯基）-21H,23H-卟啉（Thp）共固定在TiCT MXene纳米片表面，制备了一个多功能平台，生成了Pd-Thp-TiCT纳米复合材料。材料表征表明，钯纳米簇和Thp在MXene表面分布良好，同时MXene保持了其光热转换效率和宽吸收特性。在这个纳米平台中，在单一808 nm激光照射下，钯选择性催化HO分解产生O，O持续供应给Thp以增强近红外驱动的PDT。荧光和光热成像表明纳米复合材料在肿瘤部位有明显的积累。体外和体内结果均清楚地表明该纳米复合材料具有良好的生物相容性，并且协同的PTT和增强的PDT使肿瘤细胞凋亡成为可能。这项工作不仅证明了这种Pd-Thp-TiCT纳米复合材料通过协同PTT和PDT诱导肿瘤细胞凋亡，为肿瘤缺氧提供了一种有前景的解决方案，而且拓宽了有前景的光学材料在生物医学领域的应用。
