Liu Nian, O'Connor Patrick, Gujrati Vipul, Anzenhofer Pia, Klemm Uwe, Kleigrewe Karin, Sattler Michael, Plettenburg Oliver, Ntziachristos Vasilis
Chair of Biological Imaging, School of Medicine, Technical University of Munich, Munich 81675, Germany.
Institute of Biological and Medical Imaging, Helmholtz Zentrum München (GmbH), Neuherberg 85764, Germany.
Nanophotonics. 2022 Sep 26;11(21):4637-4647. doi: 10.1515/nanoph-2022-0469. eCollection 2022 Dec.
The proper design of near-infrared light-absorbing agents enables efficient optoacoustic imaging-guided phototherapy. In particular, several croconaine-based organic agents with excellent optical properties have been recently reported for this purpose. However, most of them absorb light below 800 nm, limiting deep-tissue imaging applications. To this end, we utilized a recently described novel croconaine derivative (CR880) to develop CR880-based nanoparticles (CR880-NPs) for effective delivery, deep tissue optoacoustic imaging and photothermal therapy applications. Radicals and strong - stacking in CR880 result in an 880 nm absorption peak with no blue-shift upon condensing to the solid phase. DSPE-PEG2000-formulated CR880-NPs exhibited high optoacoustic generation efficiency and photostability, and could be visualized in the tumors of three different mouse tumor models (breast, brain, and colon tumor) with high image contrast. The high photothermal conversion efficiency of CR880-NPs (∼58%) subsequently enabled efficient tumor elimination using a low energy laser, while remaining biocompatible and well-tolerated. This work introduces a promising novel agent for cancer theranostics of challenging deep-seated tumors.
近红外光吸收剂的合理设计能够实现高效的光声成像引导光疗。特别是，最近已经报道了几种具有优异光学性质的基于藏红花酸的有机试剂用于此目的。然而，它们中的大多数在800nm以下吸收光，限制了深部组织成像应用。为此，我们利用最近描述的一种新型藏红花酸衍生物（CR880）来开发基于CR880的纳米颗粒（CR880-NPs），用于有效的递送、深部组织光声成像和光热治疗应用。CR880中的自由基和强堆积导致880nm的吸收峰，在凝聚到固相时没有蓝移。DSPE-PEG2000配制的CR880-NPs表现出高光声产生效率和光稳定性，并且可以在三种不同小鼠肿瘤模型（乳腺、脑和结肠肿瘤）的肿瘤中以高图像对比度可视化。CR880-NPs的高光热转换效率（约58%）随后能够使用低能量激光有效地消除肿瘤，同时保持生物相容性和良好的耐受性。这项工作为具有挑战性的深部肿瘤的癌症诊疗引入了一种有前景的新型试剂。
