Ehrnst A
J Gen Virol. 1979 Dec;45(3):547-56. doi: 10.1099/0022-1317-45-3-547.
Fluctuations in the expression of measles virus surface-associated antigens in persistently infected human Lu 106 cells were analysed by the use of human sera which preferentially reacted with the haemolysin or haemagglutinin component. The variations observed correlated with the proportion of cells expressing surface-antigen, as examined in population analysis by indirect immunofluorescence, a radio-labelled antiglobulin technique and a cytotoxicity assay. Microfluorometric analysis revealed no changes in antigen expression at the single-cell level. The number of cells that were positive by immunofluorescence among exponentially growing, low density cells remained relatively constant. These cells were more susceptible to cytotoxicity mediated by both antisera and complement than cells seeded at high density and kept in the stationary phase. The percentage of fluorescent cells among the latter cells gradually decreased. Thus cytotoxic susceptibility was related to the proportion of the total cell population that was antigen-bearing, rather than to variations in the expression of antigen at the single-cell level. In mitotic cells, polarization of antigen, as measured by indirect immunofluorescence of pre-fixed cells, was frequently seen. Often only one of the daughter cells expressed surface antigen. The results imply that cells in stationary phase may lose antigen from their surface, possibly by shedding, and furthermore that re-expression would demand a new cell cycle.
利用优先与溶血素或血凝素成分发生反应的人血清，分析了持续感染的人Lu 106细胞中麻疹病毒表面相关抗原表达的波动情况。观察到的变化与表达表面抗原的细胞比例相关，这是通过间接免疫荧光、放射性标记抗球蛋白技术和细胞毒性试验在群体分析中检测到的。显微荧光分析显示在单细胞水平上抗原表达没有变化。在指数生长的低密度细胞中，通过免疫荧光检测呈阳性的细胞数量保持相对恒定。与接种高密度并处于静止期的细胞相比，这些细胞更容易受到抗血清和补体介导的细胞毒性作用。后一种细胞中荧光细胞的百分比逐渐降低。因此，细胞毒性易感性与携带抗原的总细胞群体比例有关，而不是与单细胞水平上抗原表达的变化有关。在有丝分裂细胞中，通过预固定细胞的间接免疫荧光测量，经常可以看到抗原的极化现象。通常只有一个子细胞表达表面抗原。结果表明，处于静止期的细胞可能会通过脱落从其表面丢失抗原，此外，重新表达需要一个新的细胞周期。
