Biyabani Arezou, Ghorbani Fereshte, Koushki Mehdi, Nedaei Keivan, Hemmati Mina, Mahdei Nasir Mahalleh Nima, Ghadimi Darya
Department of Clinical Biochemistry, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran.
Department of Medical Biotechnology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran.
Biochem Biophys Res Commun. 2025 Jan;742:151073. doi: 10.1016/j.bbrc.2024.151073. Epub 2024 Nov 29.
Throughout the recent decades, obesity has become a serious health problem that raises the risk of several diseases, including cancer, diabetes, hypertension, heart disease, neurological musculoskeletal disorders, and Non-alcoholic fatty liver disease. Some strategies, such as dietary interventions, calorie restriction (CR), and the use of antioxidant compounds, have been proposed to improve quality of life in relation to obesity. The goal of this study was to characterize the effects of CR and quercetin (QUER) on obesity-induced oxidative stress (OS). Thirty 8-week-old male BALB/c mice were divided into 5 groups of six mice each: normal diet, high-fat diet (HFD), HFD + CR, HFD + QUER (15 mg kg, IP), and HFD + QUER + CR. CR was applied as two fasting days with an interval of two days in a week. Catalase (CAT), Paraxonase 1 (PON1) and adiponectin (APN) were decreased in the HFD group, while the combination of QUER and CR increased these parameters. Treatment with QUER and CR improved Alanine transaminase and Alkaline Phosphatase enzyme activity and also the amount of leptin and insulin. Moreover, combined QUER and CR also reduced triacylglycerol (TAG), total cholesterol and TAG droplets in hepatocytes. Decreased OS was associated with the higher expression of NAD(P)H Quinone Oxidoreductase 1(NQO1) and reduced hepatic vacuoles in QUER and CR-HFD treated groups. In conclusion, these findings suggest that the combination of QUER and CR might exert protective impacts on obesity through alleviating OS and the regulation of metabolism.
在最近几十年里，肥胖已成为一个严重的健康问题，它增加了多种疾病的风险，包括癌症、糖尿病、高血压、心脏病、神经肌肉骨骼疾病和非酒精性脂肪肝病。已经提出了一些策略，如饮食干预、热量限制（CR）和使用抗氧化化合物，以改善与肥胖相关的生活质量。本研究的目的是表征CR和槲皮素（QUER）对肥胖诱导的氧化应激（OS）的影响。将30只8周龄雄性BALB/c小鼠分为5组，每组6只：正常饮食组、高脂饮食（HFD）组、HFD + CR组、HFD + QUER（15 mg/kg，腹腔注射）组和HFD + QUER + CR组。CR的实施方式为每周禁食两天，间隔两天。HFD组中过氧化氢酶（CAT）、对氧磷酶1（PON1）和脂联素（APN）降低，而QUER和CR联合使用可提高这些参数。QUER和CR治疗可改善丙氨酸转氨酶和碱性磷酸酶的活性，以及瘦素和胰岛素的含量。此外，QUER和CR联合使用还可降低肝细胞中的三酰甘油（TAG）、总胆固醇和TAG滴。OS降低与NAD(P)H醌氧化还原酶1（NQO1）的高表达以及QUER和CR-HFD治疗组中肝空泡的减少有关。总之，这些发现表明，QUER和CR联合使用可能通过减轻OS和调节代谢对肥胖产生保护作用。
