Ghorbani Fereshte, Biyabani Arezou, Ghadimi Darya, Nedaei Keivan, Khodabandehloo Hadi, Hemmati Mina
Department of Clinical Biochemistry, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran.
Department of Medical Biotechnology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran.
Int J Prev Med. 2024 Dec 28;15:74. doi: 10.4103/ijpvm.ijpvm_119_23. eCollection 2024.
Aging is caused by the progressive accumulation of various changes in the body, which is associated with an increase in free radicals and oxidative stress (OS). The aim of this study was to investigate the potential of caloric restriction (CR) and quercetin (QUER) in alleviating OS in aging and the involvement of the NAD (P) H quinone oxidoreductase 1 (NQO1)/SIRT1 signaling pathway in these effects.
Two age groups of male Wistar rats (eight and 20 weeks of age) were included in the study and subdivided into normal diet (ND), ND with QUER (15 mg Kg, IP), ND with CR, and ND with QUER and CR groups. The activities of catalase (CAT), paraoxonase (PON1), liver enzymes and lipid profiles, and the expression of SIRT1 and NQO1 genes were analyzed using the desired methods.
We showed higher liver enzymes (aspartate aminotransferase [AST], alanine transaminase [ALT], and alkaline phosphatase [ALP]), increased atherogenic lipids, and reduced PON1 activity in 20-week-old rats compared with eight-week-old rats, and the administration of QUER and CR restored these values to the normal range. The expression of NQO1 and SIRT1 is also affected by CR and QUER. CR alone and in combination with QUER significantly raised the expression of the NQO1 and SIRT1 genes. This effect was notable in SIRT1.
QUER and CR together improved the detrimental effects of aging by modulating antioxidant signaling pathways, suggesting this combination is a complementary therapeutic regime for aging and age-related diseases.
衰老由身体各种变化的渐进性积累引起，这与自由基增加和氧化应激（OS）相关。本研究的目的是探讨热量限制（CR）和槲皮素（QUER）在减轻衰老过程中氧化应激方面的潜力，以及NAD（P）H醌氧化还原酶1（NQO1）/SIRT1信号通路在这些作用中的参与情况。
本研究纳入了两个年龄组的雄性Wistar大鼠（8周龄和20周龄），并将其细分为正常饮食（ND）组、ND + QUER（15 mg/kg，腹腔注射）组、ND + CR组以及ND + QUER + CR组。使用相应方法分析过氧化氢酶（CAT）、对氧磷酶（PON1）、肝酶和血脂谱的活性，以及SIRT1和NQO1基因的表达。
我们发现，与8周龄大鼠相比，20周龄大鼠的肝酶（天冬氨酸转氨酶[AST]、丙氨酸转氨酶[ALT]和碱性磷酸酶[ALP]）更高，致动脉粥样硬化脂质增加，PON1活性降低，而给予QUER和CR可使这些值恢复到正常范围。CR和QUER也会影响NQO1和SIRT1的表达。单独的CR以及CR与QUER联合使用均显著提高了NQO1和SIRT1基因的表达。这种作用在SIRT1中尤为明显。
QUER和CR共同通过调节抗氧化信号通路改善了衰老的有害影响，表明这种联合是一种针对衰老及与年龄相关疾病的补充治疗方案。
