de Carvalho Mariana Mazetto, Ellefsen Christiane Færestrand, Eltvik Andrea Angelov, Hiorth Marianne, Samuelsen Anne Berit C
Department of Pharmacy, University of Oslo, P.O. Box 1068 Blindern, NO-136 Oslo, Norway.
Carbohydr Polym. 2025 Feb 1;349(Pt B):123021. doi: 10.1016/j.carbpol.2024.123021. Epub 2024 Nov 16.
The potential of DOSY NMR spectroscopy to distinguish the linkage pattern of chemically related polysaccharides was evaluated using β-glucans isolated from yeast (Saccharomyces cerevisiae) and mushroom (Pleurotus eryngii). Laminarin from Laminaria digitata was included for chemical shift comparison. Characterization through methylation and 1D/2D NMR analysis showed that all the samples were constituted by →3)-Glcp-(1→; →3,6)-Glcp-(1→; Glcp-(1→ and →6)-Glcp-(1→ linkages. The results obtained allowed the identification of the well-known chemical structure of laminarin. Moreover, DOSY demonstrated that the units →3)-Glcp-(1→ and →6)-Glcp-(1→ from yeast β-glucans, presented the same diffusion time. For the mushrooms β-glucans, the diffusion time of these units were different, confirming that they belong to distinct polysaccharides. The yeast and the mushroom polysaccharide samples presented the same NMR correlations, but after DOSY analysis, different structures could be proposed. Therefore, DOSY NMR spectroscopy could be a tool for the identification of different linkage patterns of polysaccharides belonging to the same group and may be a useful contribution to the chemical structure and biological activity correlation studies of such structures.
利用从酵母（酿酒酵母）和蘑菇（刺芹侧耳）中分离得到的β-葡聚糖，评估了扩散排序核磁共振光谱法（DOSY NMR）区分化学相关多糖连接模式的潜力。将来自掌状海带的海带多糖纳入其中以进行化学位移比较。通过甲基化和一维/二维核磁共振分析进行的表征表明，所有样品均由→3)-Glcp-(1→、→3,6)-Glcp-(1→、Glcp-(1→和→6)-Glcp-(1→连接组成。所得结果使得能够鉴定出海带多糖的著名化学结构。此外，DOSY表明酵母β-葡聚糖中的→3)-Glcp-(1→和→6)-Glcp-(1→单元具有相同的扩散时间。对于蘑菇β-葡聚糖，这些单元的扩散时间不同，证实它们属于不同的多糖。酵母和蘑菇多糖样品呈现出相同的核磁共振相关性，但经过DOSY分析后，可以提出不同的结构。因此，DOSY NMR光谱法可能是一种用于鉴定同一组多糖不同连接模式的工具，并且可能对这类结构的化学结构与生物活性相关性研究做出有益贡献。
