四种食用蘑菇中活性抗氧化多糖的分析。

Analyses of active antioxidant polysaccharides from four edible mushrooms.

机构信息

Jilin Province Key Laboratory on Chemistry and Biology of Changbai Mountain Natural Drugs, School of Life Sciences, Northeast Normal University, Changchun 130024, PR China.

出版信息

Int J Biol Macromol. 2019 Feb 15;123:945-956. doi: 10.1016/j.ijbiomac.2018.11.079. Epub 2018 Nov 15.

DOI:10.1016/j.ijbiomac.2018.11.079

PMID:30447375

Abstract

Four water-soluble polysaccharides were extracted from Pleurotus eryngii, Flammulina velutipes, Pleurotus ostreatus and white Hypsizygus marmoreus. Using anion exchange and gel permeation chromatography, a neutral and an acidic fraction were purified from each water-soluble polysaccharide. Their molecular weights were all around 20 kDa except that the acidic polysaccharide from Pleurotus ostreatus (named WPOPA) had a lower molecular weight of 5 kDa. Four neutral polysaccharides were mainly composed of galactose (42.7%-69.1%), followed by Man (19.4%-39.3%) and Glc (1.1%-15.9%). Four acidic polysaccharides contained glucose (59.0%-81.8%) as major sugar and minor glucuronic acid (4.5%-9.5%). Acidic polysaccharides exhibited stronger antioxidant activities than neutral fractions, and WPOPA showed the best antioxidant effects. Structural analysis indicated WPOPA had β-(1 → 6)-glucan backbone branched at O-3 by β-1,3-d-Glcp, t-β-d-Glcp and t-β-d-GlcpA. This investigation would be useful for screening natural antioxidants and significant in developing mushroom polysaccharides as functional foods.

摘要

从杏鲍菇、金针菇、平菇和白玉菇中提取了 4 种水溶性多糖。采用阴离子交换和凝胶渗透色谱法，从每种水溶性多糖中纯化出中性和酸性部分。除了来自平菇的酸性多糖（命名为 WPOPA）分子量较低，为 5 kDa 外，其他 4 种中性多糖的分子量均在 20 kDa 左右。4 种中性多糖主要由半乳糖（42.7%-69.1%）组成，其次是甘露糖（19.4%-39.3%）和葡萄糖（1.1%-15.9%）。4 种酸性多糖含有葡萄糖（59.0%-81.8%）作为主要糖，少量的葡萄糖醛酸（4.5%-9.5%）。酸性多糖的抗氧化活性强于中性部分，WPOPA 表现出最好的抗氧化效果。结构分析表明 WPOPA 具有 β-(1 → 6)-葡聚糖主链，在 O-3 位由 β-1,3-d-Glcp、t-β-d-Glcp 和 t-β-d-GlcpA 支化。这项研究对于筛选天然抗氧化剂具有重要意义，并有助于将菇类多糖开发为功能性食品。