Jiang Chunyan, Zhao Xiaofeng
Department of Gastrointestinal Tumor Surgery, Xingtai People's Hospital, Xingtai, China.
Turk J Gastroenterol. 2024 Nov 28;35(12):889-899. doi: 10.5152/tjg.2024.23669.
BACKGROUND/AIMS: Colorectal cancer (CRC) is a widespread cancerous disease with an unfavorable prognosis. MIR210HG appears to have a significant connection with the development of CRC, but the precise regulatory mechanism remains obscure.
Quantitative real-time polymerase chain reaction was utilized to determine expression quantities of MIR210HG and miR-1226-3p. The proliferative capacity of CRC cells was measured by cell counting kit-8. The apoptosis rate of cells was examined using flow cytometry. The invasive capability was assessed through the transwell experiment. The targeted regulation of MIR210HG and miR-1226-3p was validated through dual-luciferase reporter gene experiments.
In carcinoma tissues and blood serum of colorectal cancer patients and cell lines, MIR210HG expression was upregulated, while the miR-1226-3p expression was downregulated. MIR210HG had a diagnostic and prognostic value for CRC patients. MIR210HG may target and regulate miR-1226-3p. MIR210HG may have the capacity to augment the vitality and invasion of CRC cells and suppress cell apoptosis, and this influence is counteracted by miR-1226-3p.
lncRNA MIR210HG accelerated the progression of colorectal cancer by controlling miR-1226-3p. lncRNA MIR210HG/miR1226-3p may potentially serve as therapeutic targets for addressing colorectal cancer.
背景/目的：结直肠癌（CRC）是一种广泛存在且预后不良的癌症疾病。MIR210HG似乎与CRC的发生发展存在显著关联，但其确切调控机制仍不清楚。
采用定量实时聚合酶链反应来测定MIR210HG和miR - 1226 - 3p的表达量。通过细胞计数试剂盒 - 8检测CRC细胞的增殖能力。使用流式细胞术检测细胞凋亡率。通过Transwell实验评估侵袭能力。通过双荧光素酶报告基因实验验证MIR210HG与miR - 1226 - 3p的靶向调控关系。
在结直肠癌患者的癌组织、血清以及细胞系中，MIR210HG表达上调，而miR - 1226 - 3p表达下调。MIR210HG对CRC患者具有诊断和预后价值。MIR210HG可能靶向调控miR - 1226 - 3p。MIR210HG可能具有增强CRC细胞活力和侵袭能力以及抑制细胞凋亡的作用，而这种影响可被miR - 1226 - 3p抵消。
lncRNA MIR210HG通过调控miR - 1226 - 3p加速了结直肠癌的进展。lncRNA MIR210HG/miR1226 - 3p可能有望成为治疗结直肠癌的靶点。
