长链非编码 MIR210HG 的敲低通过 microRNA-608-FOXO6 轴抑制肝癌细胞的增殖、迁移和侵袭。

Knockdown of long non-coding MIR210HG inhibits cell proliferation, migration, and invasion in hepatoblastoma via the microRNA-608-FOXO6 axis.

机构信息

Department of Pediatric Surgery, The Affiliated Hospital of Qingdao University, Qingdao, China.

Department of Pediatric Surgery, Qingdao Women and Children's Hospital, Qingdao, China.

出版信息

J Int Med Res. 2021 Dec;49(12):3000605211054695. doi: 10.1177/03000605211054695.

OBJECTIVE

Hepatoblastoma is the most common liver tumor. Recent research has found that long non-coding (lnc)RNAs are involved in multiple types of cancers, but the potential mechanism of lncRNA MIR210HG in hepatoblastoma remains unknown. The present study explored the molecular mechanism of MIR210HG in hepatoblastoma progression.

METHODS

The cell counting kit-8 was used to detect cell viability, and Transwell assays assessed cell migration and invasion. Luciferase reporter assays showed the relationship between MIR210HG and microRNA (miR)-608 and between miR-608 and forkhead box O6 (FOXO6). Functional tests were verified by a tumor xenograft model. The expression of MIR210HG, miR-608, FOXO6, E-cadherin, N-cadherin, and vimentin was determined by quantitative reverse transcription polymerase chain reaction and western blotting.

RESULTS

MIR210HG was shown to be highly expressed in hepatoblastoma tissues and cell lines. Knockdown of MIR210HG reduced proliferation, migration, and invasion in liver cancer cells, and suppressed tumor growth . MIR210HG competitively combined with miR-608, and miR-608 decreased FOXO6 expression.

CONCLUSION

Our study demonstrated that knockdown of MIR210HG inhibits hepatoblastoma development through binding to miR-608 and downregulating FOXO6. Our results provide novel insights for hepatoblastoma treatment involving the MIR210HG-miR608-FOXO6 axis.

目的

肝细胞瘤是最常见的肝脏肿瘤。最近的研究发现，长链非编码（lnc）RNAs 参与多种类型的癌症，但 lncRNA MIR210HG 在肝细胞瘤中的潜在机制尚不清楚。本研究探讨了 MIR210HG 在肝细胞瘤进展中的分子机制。

方法

使用细胞计数试剂盒-8 检测细胞活力，Transwell 分析评估细胞迁移和侵袭。荧光素酶报告实验显示了 MIR210HG 与 microRNA（miR）-608 之间以及 miR-608 与叉头框 O6（FOXO6）之间的关系。肿瘤异种移植模型验证了功能测试。通过定量逆转录聚合酶链反应和蛋白质印迹法测定 MIR210HG、miR-608、FOXO6、E-钙黏蛋白、N-钙黏蛋白和波形蛋白的表达。