Xu Muxin, Ma Yanyan, Zhao Yuping, Xu Lizhen, Lin Weiying
Institute of Optical Materials and Chemical Biology, Guangxi Key Laboratory of Electrochemical Energy Materials, School of Chemistry and Chemical Engineering, Guangxi University, Nanning, Guangxi, 530004, China.
Department of Otolaryngology-Head and Neck Surgery, Shandong Institute of Otorhinolaryngology, Shandong Provincial ENT Hospital, Shandong University, Jinan, 250022, China.
Anal Chim Acta. 2025 Jan 15;1335:343443. doi: 10.1016/j.aca.2024.343443. Epub 2024 Nov 19.
Peroxynitrite (ONOO) is a bioactive molecule involved in various biochemical processes, and the abnormal concentration fluctuations of ONOO in living systems are closely associated with various diseases, including cancer. An important characteristic of the tumor microenvironment is the overexpression of ONOO, highlighting the significance of specific detection of ONOO in distinguishing between tumor tissue and normal tissue. A single near-infrared second window (NIR-II) molecular probe integrated fluorescence imaging and photothermal therapy can achieve precise localization and effective ablation of deep-seated tumor tissue. However, it still remains challenges. (87) RESULTS: In this study, we present a probe (BDⅡ-ONOO) that integrates NIR-II fluorescence imaging and photothermal therapy for the specific detection of ONOO. The probe exhibits excellent selectivity, high sensitivity, low toxicity and high biocompatibility. In the presence of ONOO, the probe can quickly respond to ONOO and emit NIR-II fluorescence at 900 nm with 7.6-fold change in fluorescence intensity. In addition, the probe BDⅡ-ONOO exhibits a high photothermal conversion efficiency of 41.6 % under 808 nm laser irradiation in the presence of ONOO. In vivo imaging results indicate that the probe BDⅡ-ONOO not only effectively distinguishes tumor tissue from normal tissue but also performs photothermal treatment on 4T1 tumor without apparent biological toxicity. (112) SIGNIFICANCE: This work provides insights for the future development of tumor-specific diagnostic and therapeutic approaches with good biocompatibility and deep tissue penetration. The probe described in this work may be employed as a powerful tool for the diagnosis and precise treatment of tumors in vivo. (44).
过氧亚硝酸根（ONOO）是一种参与多种生化过程的生物活性分子，其在生物系统中的异常浓度波动与包括癌症在内的多种疾病密切相关。肿瘤微环境的一个重要特征是ONOO的过表达，这突出了特异性检测ONOO在区分肿瘤组织和正常组织方面的重要性。一种集成了荧光成像和光热疗法的近红外二区（NIR-II）分子探针能够实现对深部肿瘤组织的精确定位和有效消融。然而，这仍然存在挑战。（87）结果：在本研究中，我们展示了一种用于特异性检测ONOO的集成NIR-II荧光成像和光热疗法的探针（BDⅡ-ONOO）。该探针具有优异的选择性、高灵敏度、低毒性和高生物相容性。在ONOO存在的情况下，该探针能够快速响应ONOO，并在900 nm处发射NIR-II荧光，荧光强度变化7.6倍。此外，在ONOO存在的情况下，探针BDⅡ-ONOO在808 nm激光照射下表现出41.6%的高光热转换效率。体内成像结果表明，探针BDⅡ-ONOO不仅能有效区分肿瘤组织和正常组织，还能对4T1肿瘤进行光热治疗，且无明显生物毒性。（112）意义：这项工作为未来开发具有良好生物相容性和深部组织穿透性的肿瘤特异性诊断和治疗方法提供了思路。本文所述的探针可作为体内肿瘤诊断和精确治疗的有力工具。（44）
