Zhao Ke-Yu, Du Yi-Xiang, Cao Hui-Min, Su Li-Ya, Su Xiu-Lan, Li Xian
Key Laboratory of Medical Cell Biology in Inner Mongolia, Clinical Medical Research Center, Affiliated Hospital of Inner Mongolia Medical University, Hohhot, Inner Mongolia 010050, China; Key Laboratory of Medical Cell Biology in Inner Mongolia, Inner Mongolia Bioactive Peptide Engineering Laboratory, 1 North Tongdao Street, Hohhot, Inner Mongolia 010050, China.
Inner Mongolia Medical University, Hohhot, Inner Mongolia 010050, China.
Colloids Surf B Biointerfaces. 2025 Mar;247:114435. doi: 10.1016/j.colsurfb.2024.114435. Epub 2024 Dec 6.
Matrigel is the most commonly used matrix for 3D organoid cultures. Research on the biomaterial basis of Matrigel for organoid cultures is a highly challenging field. Currently, many studies focus on Matrigel-based biological macromolecules or combinations to construct natural Matrigel and synthetic hydrogel scaffolds based on collagen, peptides, polysaccharides, microbial transglutaminase, DNA supramolecules, and polymers for organoid culture. In this review, we discuss the limitations of both natural and synthetic Matrigel, and describe alternative scaffolds that have been employed for organoid cultures. The patient-derived organoids were constructed in different cancer types and limitations of animal-derived organoids based on the hydrogel or Matrigel. The constructed techniques utilizing 3D bioprinting platforms, air-liquid interface (ALI) culture, microfluidic culture, and organ-on-a-chip platform are summarized. Given the potential of organoids for a wide range of therapeutic, tissue engineering and pharmaceutical applications, it is indeed imperative to develop defined and customized hydrogels in addition to Matrigel.
基质胶是三维类器官培养中最常用的基质。关于用于类器官培养的基质胶的生物材料基础的研究是一个极具挑战性的领域。目前，许多研究聚焦于基于基质胶的生物大分子或其组合，以构建基于胶原蛋白、肽、多糖、微生物转谷氨酰胺酶、DNA超分子和聚合物的天然基质胶和合成水凝胶支架用于类器官培养。在本综述中，我们讨论了天然和合成基质胶的局限性，并描述了已用于类器官培养的替代支架。基于水凝胶或基质胶构建了不同癌症类型的患者来源类器官以及动物来源类器官的局限性。总结了利用三维生物打印平台、气液界面（ALI）培养、微流控培养和芯片器官平台的构建技术。鉴于类器官在广泛的治疗、组织工程和药物应用中的潜力，除了基质胶之外，开发明确且定制的水凝胶确实势在必行。
