Patil P P, Nagy K, ÁBrahám Á, Vass I, Szabó M
Institute of Plant Biology, HUN-REN Biological Research Centre, 6726 Szeged, Hungary.
Institute of Biophysics, HUN-REN Biological Research Centre, 6726 Szeged, Hungary.
Photosynthetica. 2023 Dec 18;61(4):473-482. doi: 10.32615/ps.2023.042. eCollection 2023.
is an important species of green algae because it produces the high-value carotenoid astaxanthin. Astaxanthin production is enhanced by various stress conditions causing the transformation of green vegetative cells to red cells with high amounts of astaxanthin, which plays various photoprotective and antioxidant roles. Although intensive research has been conducted to reveal the regulation of astaxanthin production, the photosynthetic capacity of the various cell forms is unresolved at the single-cell level. In this work, we characterized the photosynthetic and morphological changes of cells, using a combination of microfluidic tools and microscopic chlorophyll fluorescence imaging. We found marked but reversible changes in the variable chlorophyll fluorescence signatures upon the transformation of green cells to red cells, and we propose that the photosynthetic activity as revealed by single-cell chlorophyll fluorescence kinetics serves as a useful phenotypic marker of the different cell forms of .
是绿藻的一个重要物种，因为它能产生高价值的类胡萝卜素虾青素。各种胁迫条件会增强虾青素的产生，导致绿色营养细胞转变为富含大量虾青素的红色细胞，虾青素发挥着各种光保护和抗氧化作用。尽管已经进行了深入研究以揭示虾青素产生的调控机制，但在单细胞水平上，各种细胞形式的光合能力仍未得到解决。在这项工作中，我们结合微流控工具和显微镜叶绿素荧光成像，对细胞的光合和形态变化进行了表征。我们发现，绿色细胞转变为红色细胞时，可变叶绿素荧光特征会发生显著但可逆的变化，并且我们提出，单细胞叶绿素荧光动力学所揭示的光合活性可作为不同细胞形式的有用表型标记。
