Low humidity enhances thermotolerance in .

Humidity is an important environmental factor that causes physiological changes in organisms. In humans, high humidity disrupts thermoregulation by limiting heat dissipation, leading to heat stress. While lacks comparable thermoregulatory systems, humidity may still impact its heat tolerance by affecting cellular stress responses. We tested this by subjecting to heat shock under different humidity conditions and found that lower humidity during heat shock improved survival compared to higher humidity. These findings demonstrate that humidity is an important variable affecting thermotolerance in and should be standardized in heat-stress experiments.