Albayrak Omercan, Hatipoglu Bilge Nur, Ozbeyli Dilek, Sen Ali, Koyuncuoglu Turkan, Cevik Ozge, Ercan Feriha, Kanpalta Fatma, Dogan Ahmet, Pazarbasi Seren Ede, Sener Goksel
Department of Research and Development, Anatolia Geneworks, Istanbul, Turkiye.
Department of Pharmacology, Marmara University Faculty of Pharmacy, Istanbul, Turkiye.
North Clin Istanb. 2024 Nov 14;11(6):512-519. doi: 10.14744/nci.2023.95776. eCollection 2024.
In our study, the protective effect of dodder plant extract against encephalopathy induced by cholestatic liver disease model was investigated.
Spraque Dawley rats were used in the study. For the cholestatic liver disease model, the bile duct ligation (BDL) was applied. The groups were determined as control, Cuscuta sp. (CUS), BDL and BDL + CUS. Double ligation was performed in the bile duct in the BDL groups. For the applications, saline (SF) was administered to the control and BDL groups for 28 days while 250 mg/kg of Cuscuta sp. extract was given by oral gavage to the CUS and BDL + CUS groups. At the end of the experiment, cognitive evaluations were made by applying new object recognition and Morris water maze tests. After these tests, blood-brain barrier (BBB) measurements were made in half of the groups. In the other half of the groups, brain tissue samples were taken by decapitation and transforming growth factor-beta (TGF-β), 8-hydroxydeoxyguanosine (8-OHdG) and sodium-potassium adenosine triphosphatase (Na/K-ATPase) measurements were made in the tissues. Histological examinations of the tissues were also performed.
Cognitive performance was low, and BBB permeability was found to be increased in the group with bile duct ligation. In addition, TGF-β and 8-OHdG levels were increased in tissues, while Na/K-ATPase enzyme activity was suppressed. Treatment with Cuscuta sp. increased cognitive performance and decreased BBB permeability. Other biochemical parameters examined were significantly (p<0.05-0.001) reversed and supported by histological findings.
Our findings in the study suggest that dodder plant may be beneficial for the protection of cognitive performance and brain tissue in encephalopathy caused by cholestasis.
在本研究中，探讨菟丝子植物提取物对胆汁淤积性肝病模型所致脑病的保护作用。
本研究使用Spraque Dawley大鼠。对于胆汁淤积性肝病模型，采用胆管结扎（BDL）法。将大鼠分为对照组、菟丝子组（CUS）、BDL组和BDL + CUS组。BDL组在胆管进行双重结扎。给药方面，对照组和BDL组给予生理盐水（SF），持续28天，而CUS组和BDL + CUS组通过口服灌胃给予250 mg/kg的菟丝子提取物。实验结束时，通过新物体识别和莫里斯水迷宫试验进行认知评估。这些试验后，对一半的组进行血脑屏障（BBB）测量。在另一半组中，通过断头采集脑组织样本，并在组织中测量转化生长因子-β（TGF-β）、8-羟基脱氧鸟苷（8-OHdG）和钠钾三磷酸腺苷酶（Na/K-ATPase）。还对组织进行了组织学检查。
胆管结扎组认知能力低下，血脑屏障通透性增加。此外，组织中TGF-β和8-OHdG水平升高，而Na/K-ATPase酶活性受到抑制。菟丝子治疗可提高认知能力，降低血脑屏障通透性。其他检测的生化参数有显著改善（p<0.05 - 0.001），并得到组织学结果的支持。
我们的研究结果表明，菟丝子植物可能对保护胆汁淤积所致脑病中的认知能力和脑组织有益。
