Nandanwar Nishant, Gu Geoffery, Gibson Joy E, Neely Michael N
Division of Infectious Diseases, Department of Pediatrics, Children's Hospital Los Angeles, Los Angeles, CA, United States.
Department of Biological Sciences, University of Southern California, Los Angeles, CA, United States.
Front Microbiol. 2024 Nov 25;15:1484510. doi: 10.3389/fmicb.2024.1484510. eCollection 2024.
The lungs of patients with cystic fibrosis (CF) are vulnerable to persistent polymicrobial colonization by bacterial pathogens including , and the non-tuberculous mycobacterium (NTM) . The polymicrobial milieu within the CF lung impacts individual species fitness, influences biofilm-forming capabilities, pathogenicity, production of virulence factors and even antimicrobial responses, all potentially compromising therapeutic success. Interaction studies among these CF pathogens are very limited, especially studies on the influences of and on co-existence and virulence. Based on the little known thus far about coinfection of these pathogens, we hypothesize that the co-existence of and alters virulence and phenotypic characteristics. We evaluated the direct (co-culture) and indirect (using supernatant) effects of and on growth rate, biofilm formation, macrophage internalization and glycopeptidolipids (GPL) expression. Our observations indicate that and exert a competitive behavior toward during direct contact or indirect interaction , probably as is the case of polymicrobial infections in the lungs of patients with CF. This is the first report that demonstrates inhibitory effects on growth and biofilm forming capabilities. Collectively, co-culture studies enhance our understanding of polymicrobial interactions during coinfection and can guide to establish better management of coinfections and treatment strategies for .
囊性纤维化（CF）患者的肺部易受到包括[具体细菌名称1]和非结核分枝杆菌（NTM）[具体细菌名称2]在内的多种细菌病原体的持续多微生物定植。CF肺部的多微生物环境会影响单个菌种的适应性，影响生物膜形成能力、致病性、毒力因子的产生甚至抗菌反应，所有这些都可能危及治疗效果。这些CF病原体之间的相互作用研究非常有限，尤其是关于[具体细菌名称1]和[具体细菌名称2]对[具体细菌名称3]共存和毒力影响的研究。基于目前对这些病原体共感染的了解甚少，我们假设[具体细菌名称1]和[具体细菌名称2]的共存会改变[具体细菌名称3]的毒力和表型特征。我们评估了[具体细菌名称1]和[具体细菌名称2]对[具体细菌名称3]的生长速率、生物膜形成、巨噬细胞内化和糖脂（GPL）表达的直接（共培养）和间接（使用上清液）影响。我们的观察结果表明，在直接接触或间接相互作用期间，[具体细菌名称1]和[具体细菌名称2]对[具体细菌名称3]表现出竞争行为，这可能与CF患者肺部的多微生物感染情况相同。这是第一份证明[具体细菌名称1]对[具体细菌名称3]生长和生物膜形成能力具有抑制作用的报告。总的来说，共培养研究增强了我们对共感染期间多微生物相互作用的理解，并可以指导建立更好的共感染管理和[具体细菌名称3]的治疗策略。
