Li Jingjing, Liang Aihui, Wen Guiqing, Jiang Zhiliang
State Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources, School of Chemistry and Pharmaceutical Sciences, Guangxi Normal University, Guilin 541004, China; Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology, Guilin 541004, China; Guangxi Key Laboratory of Environmental Processes and Remediation in Ecologically Fragile Regions, Guilin 541004, China.
State Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources, School of Chemistry and Pharmaceutical Sciences, Guangxi Normal University, Guilin 541004, China; Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology, Guilin 541004, China; Guangxi Key Laboratory of Environmental Processes and Remediation in Ecologically Fragile Regions, Guilin 541004, China.
J Colloid Interface Sci. 2025 Mar 15;682:946-960. doi: 10.1016/j.jcis.2024.12.016. Epub 2024 Dec 6.
A new nanopalladium surface molecularly imprinted covalent organic framework (MICOF) catalytic probe (Pd@TpPa) for enrofloxacin (ENR) was synthesized by molecular imprinting technology, using 1,3,5-triformylphloroglucinol (Tp) and p-phenylenediamine (Pa) as monomers, ENR as the template molecule, and palladium nanoparticles (PdNP) as the core of nanocatalytic probe. This nanoprobe not only specifically recognizes ENR but also catalyzes the cupric tartrate-glucose (GL) indicator reaction. The amino groups in TpPa replace the tartrate ions, forming a new complex with Cu. This new complex enhances the efficiency of GL oxidation reaction. The generated CuO nanoparticles exhibit strong resonance Rayleigh scattering (RRS) and absorption (Abs) peaks at 370 nm and 500 nm, respectively. The catalytic and analytical performances of metal nanoparticles including Pd, Pt, Au and Ag were studied. It was found that Pd@TpPa exhibited the best performance. The RRS response was linear to ENR concentration in the range of 0.01-1.5 nM, with a detection limit of 0.008 nM ENR. The method was applied to analyze river water samples, yielding recoveries of 96.9-106.6 % and precision between 2.33-8.49 %. This nanocatalytic probe-RRS analysis platform is simple, sensitive, selective, and versatile, making it applicable for the determination of norfloxacin (NOR), ofloxacin, pefloxacin, fleroxacin, and ciprofloxacin, with linear ranges of 0.02-6.0 nM, 0.04-6.0 nM, 0.04-6.0 nM, 0.1-15 nM, and 0.1-15 nM, respectively.
采用分子印迹技术，以1,3,5-三(4-甲酰基苯基)苯（Tp）和对苯二胺（Pa）为单体，恩诺沙星（ENR）为模板分子，钯纳米颗粒（PdNP）为纳米催化探针核心，合成了一种用于检测恩诺沙星的新型纳米钯表面分子印迹共价有机框架（MICOF）催化探针（Pd@TpPa）。该纳米探针不仅能特异性识别恩诺沙星，还能催化酒石酸铜-葡萄糖（GL）指示反应。TpPa中的氨基取代酒石酸根离子，与铜形成新的配合物。这种新配合物提高了GL氧化反应的效率。生成的氧化铜纳米颗粒在370 nm和500 nm处分别表现出强烈的共振瑞利散射（RRS）和吸收（Abs）峰。研究了包括Pd、Pt、Au和Ag在内的金属纳米颗粒的催化和分析性能。结果发现Pd@TpPa表现出最佳性能。RRS响应与0.01 - 1.5 nM范围内的恩诺沙星浓度呈线性关系，恩诺沙星的检测限为0.008 nM。该方法用于分析河水样品，回收率为96.9 - 106.6%，精密度在2.33 - 8.49%之间。这种纳米催化探针-RRS分析平台简单、灵敏、选择性好且通用性强，适用于诺氟沙星（NOR）、氧氟沙星、培氟沙星、氟罗沙星和环丙沙星的测定，线性范围分别为0.02 - 6.0 nM、0.04 - 6.0 nM、0.04 - 6.0 nM、0.1 - 15 nM和0.1 - 15 nM。
