Wang Kai, Yan Xiaohai, Wu Jiamin, Qi Jianrui, Ning Mengge, Li Menghui, Sun Rui, Wang Zhouli, Yuan Yahong, Yue Tianli
College of Food Science and Engineering, Northwest A&F University, Yangling 712100, Shaanxi, China; Laboratory of Quality & Safety Risk Assessment for Agro-products (Yangling), Ministry of Agriculture, Yangling 712100, China.
College of Food Science and Technology, Northwest University, Xi'an 710067, China.
Food Chem. 2025 Mar 1;467:142365. doi: 10.1016/j.foodchem.2024.142365. Epub 2024 Dec 6.
Deoxynivalenol (DON) contamination in cereals is a widespread issue with global implications, necessitating the development of efficient detection methods. Here, a fluorescent aptasensor integrating target-responsive DNA three-way junction (TWJ) and DNA walking machine was developed to detect DON. The DON-specific aptamer (Apt) and the walker (Walker DNA) are integrated into TWJs. TWJs initiate the DNA walking machine with the assistance of Nb.BbvCI to provide signal amplification for sensing events. The constructed fluorescent aptasensor selectively recognizes and detects DON. Additionally, the sensing platform explored the quantitative relationship between fluorescence signal changes and DON concentration, achieving a limit of detection (LOD) of 0.009 ng/mL. Ultimately, the method performed well in the detection of cornmeal samples with recoveries ranging from 95.49 % to 100.82 %. The method demonstrates good stability and is expected to be a potential candidate for the accurate detection of DON in grains.
谷物中的脱氧雪腐镰刀菌烯醇（DON）污染是一个具有全球影响的普遍问题，因此需要开发高效的检测方法。在此，开发了一种集成了靶标响应性DNA三向连接体（TWJ）和DNA步行机的荧光适配体传感器来检测DON。DON特异性适配体（Apt）和步行者（步行者DNA）被整合到TWJs中。TWJs在Nb.BbvCI的协助下启动DNA步行机，为传感事件提供信号放大。构建的荧光适配体传感器能够选择性地识别和检测DON。此外，该传感平台探索了荧光信号变化与DON浓度之间的定量关系，实现了0.009 ng/mL的检测限（LOD）。最终，该方法在玉米粉样品检测中表现良好，回收率在95.49%至100.82%之间。该方法具有良好的稳定性，有望成为谷物中DON准确检测的潜在候选方法。
