Wu Jinlei, Yuan Haojun, Yang Yunhua, Yang Ping, Yan Xinxin, Mu Ying, Jin Qinghui, Yang Panhui, Gao Wanlei
College of Information Science and Engineering, Ningbo University, Ningbo 315211, Zhejiang, China.
Mechanical and Electrical Engineering School, Zhejiang Fashion Institute of Technology, Ningbo 315211, Zhejiang, China.
Food Chem. 2025 May 1;473:143072. doi: 10.1016/j.foodchem.2025.143072. Epub 2025 Jan 26.
Mycotoxins are widely found as co-contaminants in agricultural products and their derivatives. However, on-site monitoring of multiple mycotoxins remains a significant challenge. We developed an electrochemical aptasensor integrating microelectrodes and microfluidics for simultaneous aflatoxin B1 (AFB1) and deoxynivalenol (DON) detection. Its sensing interface had dual working electrodes modified with two specific aptamers, sharing a counter and a reference electrode. The microfluidic chip's comb-shaped structure enhanced aptamer-target binding. Using a buffer with redox probes for electrochemical detection, we achieve label-free quantitative detection of both toxins within 20 min. The sensor had detection limits of 7.74 pg/mL for AFB1 and 0.172 ng/mL for DON, with linear detection ranges of 0.03-300 ng/mL and 0.3-3000 ng/mL respectively. It exhibited excellent specificity, repeatability and stability. In summary, this electrochemical aptasensor enables rapid and sensitive on-site detection of AFB1 and DON, safeguarding food safety during grain storage for early monitoring and prevention.
霉菌毒素作为共污染物广泛存在于农产品及其衍生物中。然而，对多种霉菌毒素进行现场监测仍然是一项重大挑战。我们开发了一种集成微电极和微流体的电化学适体传感器，用于同时检测黄曲霉毒素B1（AFB1）和脱氧雪腐镰刀菌烯醇（DON）。其传感界面有两个工作电极，分别用两种特异性适体修饰，共用一个对电极和一个参比电极。微流控芯片的梳状结构增强了适体与靶标的结合。使用含有氧化还原探针的缓冲液进行电化学检测，我们在20分钟内实现了对两种毒素的无标记定量检测。该传感器对AFB1的检测限为7.74 pg/mL，对DON的检测限为0.172 ng/mL，线性检测范围分别为0.03 - 300 ng/mL和0.3 - 3000 ng/mL。它具有优异的特异性、重复性和稳定性。总之，这种电化学适体传感器能够快速、灵敏地现场检测AFB1和DON，在粮食储存期间保障食品安全，实现早期监测和预防。
