采用气相色谱-质谱联用技术测定人血浆中6-氧代前列环素F1α的含量。

Measurement of 6-oxo-PGF1 alpha in human plasma using gas chromatography-mass spectrometry.

作者信息

Hensby C N, Fitzgerald G A, Friedman L A, Lewis P J, Dollery C T

出版信息

Prostaglandins. 1979 Nov;18(5):731-6. doi: 10.1016/0090-6980(79)90093-5.

Abstract

The plasma concentration of the prostacyclin (PGI2) hydration product 6-oxo-PGF1 alpha has been assayed by stable isotope dilution GC-MS in six normal volunteers infused with increasing doses of PGI2 intravenously. The predosing levels of 6-oxo-PGF1 alpha ranged between 114 and 266 pg/ml. Infusion of PGI2 increased 6-oxo-PGF1 alpha concentration in plasma but the increments were lower than expected suggesting less conversion of the PGI2 to 6-oxo-PGF1 alpha at high infusion rates.

摘要

通过稳定同位素稀释气相色谱-质谱法测定了6名正常志愿者静脉注射递增剂量前列环素（PGI2）后血浆中前列环素（PGI2）水合产物6-氧代-PGF1α的浓度。给药前6-氧代-PGF1α的水平在114至266 pg/ml之间。注射PGI2可使血浆中6-氧代-PGF1α浓度升高，但升高幅度低于预期，提示在高注射速率下PGI2向6-氧代-PGF1α的转化较少。