Yang Guang, Mo Hongjuan, Liu Bingjie, Wu Yiwen, Liu Guiqin, Hu Yadong, Jiao Xue, Guo Kexin, Wei Xin, Fang Yaowei, Pan Mingxuan, Hao Liang
Jiangsu Key Laboratory of Marine Bioresources and Environment, Co-Innovation Center of Jiangsu Marine Bio-industry Technology, School of Ocean Food and Biological Engineering, Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Marine Resources Development Research Institute, Jiangsu Ocean University, Lianyungang 222005, China.
Jiangsu Key Laboratory of Marine Bioresources and Environment, Co-Innovation Center of Jiangsu Marine Bio-industry Technology, School of Ocean Food and Biological Engineering, Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Marine Resources Development Research Institute, Jiangsu Ocean University, Lianyungang 222005, China.
Int J Biol Macromol. 2025 Jan;286:138544. doi: 10.1016/j.ijbiomac.2024.138544. Epub 2024 Dec 8.
Natural polymer hydrogels, such as pullulan-based hydrogels, offer significant advantages over synthetic materials due to their thermal stability, film-forming capacity, solubility, adhesiveness, and antioxidant properties. In this study, the strain Aureobasidium melanogenum TZ-FC3, which produces a high level of pullulan, was successfully isolated from the mangrove ecosystems of Guangdong Province, China. 66.01 ± 1.10 g/L pullulan without melanin was produced by the TZ-FC3 strain within 120 h at flask level. Pullulan fermented by A. melanogenum TZ-FC3 was added to enhance the hydrogen bond network within the pullulan/PVA/borax hydrogels (P-2, P-3 and P-4 hydrogels) to improve mechanical strength and crosslinking density of PVA/borax hydrogel (P-1 hydrogel). Compared to the P-1 hydrogel, the P-2 hydrogel exhibited a 65.4 % increase in tensile strain, a remarkable 694.03 % increase in tensile strength and improved the degree of internal crosslinking. Additionally, the pullulan/PVA/borax hydrogels demonstrated excellent self-healing properties, adhesion, injectability, and antibacterial activity. The preparation process of pullulan/PVA/borax hydrogels is straightforward and effective, suggesting broad industrial applicability and underscoring their potential as next-generation materials for advanced healthcare solutions.
天然聚合物水凝胶，如基于普鲁兰多糖的水凝胶，由于其热稳定性、成膜能力、溶解性、粘附性和抗氧化性能，与合成材料相比具有显著优势。在本研究中，从中国广东省的红树林生态系统中成功分离出了高产普鲁兰多糖的产黑素 Aureobasidium melanogenum TZ-FC3 菌株。TZ-FC3 菌株在摇瓶水平下 120 小时内可产生 66.01±1.10 g/L 不含黑色素的普鲁兰多糖。添加由产黑素 Aureobasidium melanogenum TZ-FC3 发酵的普鲁兰多糖以增强普鲁兰多糖/PVA/硼砂水凝胶（P-2、P-3 和 P-4 水凝胶）中的氢键网络，从而提高 PVA/硼砂水凝胶（P-1 水凝胶）的机械强度和交联密度。与 P-1 水凝胶相比, P-2 水凝胶的拉伸应变增加了 65.4%，拉伸强度显著增加了 694.03%，并提高了内部交联程度。此外，普鲁兰多糖/PVA/硼砂水凝胶还表现出优异的自愈性能、粘附性、可注射性和抗菌活性。普鲁兰多糖/PVA/硼砂水凝胶的制备过程简单有效，表明其具有广泛的工业适用性，并突出了它们作为先进医疗保健解决方案的下一代材料的潜力。
