Bartels Nomi, Argyropoulou Aikaterini, Al-Ahmad Ali, Hellwig Elmar, Skaltsounis Alexios Leandros, Wittmer Annette, Vach Kirstin, Karygianni Lamprini
Department of Prosthodontics, Faculty of Medicine Carl Gustav Carus, TU Dresden, Dresden, Germany.
Department of Pharmacognosy and Natural Products Chemistry, Faculty of Pharmacy, National and Kapodistrian University of Athens, Athens, Greece.
Front Dent Med. 2025 Apr 4;6:1535753. doi: 10.3389/fdmed.2025.1535753. eCollection 2025.
A range of disinfectant mouthwashes are available for oral hygiene. The gold standard is Chlorhexidine digluconate (CHX), which, like other available products, cannot be used without side effects in the long term. However, in recent years, therapy with herbal products, often considered antiquated, has regained considerable interest. Therefore, the search for plant compounds as an alternative to existing oral disinfectants is meaningful.
In this study, eleven Mediterranean plant extracts were tested for their antimicrobial effect . Methanol extracts of the following plants were produced by the pharmaceutical faculty of the University of Athens: , , , , , , , , , , and . The extracts were dissolved for microdilution experiments at concentrations ranging from 10 to 0.019 mg/ml. The oral pathogens tested were , , , , , , , and . , , and were used as references.
All extracts, except the methanol extract of , showed an antibacterial effect at concentrations ranging from 10 to 0.15 mg/ml. None of the extracts exhibited a significant antifungal effect. In general, the anaerobic pathogens could be inhibited and killed at lower concentrations compared to the aerobic pathogens. also showed good susceptibility to the extracts. Additionally, the extracts' ability to inhibit biofilm formation by was tested. at a concentration of 0.3 mg/ml showed a moderate inhibitory effect. The extracts of , , , and the methanol extract of were effective at concentrations up to 1.25 mg/ml. was able to inhibit and kill at a concentration of 0.6 mg/ml, but its effectiveness in biofilm inhibition significantly decreased at 2.5 mg/ml.
The study's hypothesis that all extracts would exhibit an antimicrobial effect was thus confirmed.
有一系列用于口腔卫生的消毒漱口水。金标准是葡萄糖酸氯己定（CHX），与其他现有产品一样，长期使用会有副作用。然而，近年来，常被认为过时的草药产品疗法重新引起了人们的极大兴趣。因此，寻找植物化合物作为现有口腔消毒剂的替代品是有意义的。
在本研究中，测试了11种地中海植物提取物的抗菌效果。雅典大学药学院制备了以下植物的甲醇提取物：[此处原文缺失植物名称]。提取物溶解后用于微稀释实验，浓度范围为10至0.019毫克/毫升。测试的口腔病原体有[此处原文缺失病原体名称]。[此处原文缺失作为参考的物质名称]用作参考。
除[此处原文缺失植物名称]的甲醇提取物外，所有提取物在浓度为10至0.15毫克/毫升时均显示出抗菌作用。没有提取物表现出显著的抗真菌作用。一般来说，与需氧病原体相比，厌氧病原体在较低浓度下即可被抑制和杀灭。[此处原文缺失病原体名称]对提取物也表现出良好的敏感性。此外，还测试了提取物抑制[此处原文缺失病原体名称]形成生物膜的能力。浓度为0.3毫克/毫升的[此处原文缺失物质名称]显示出中等抑制作用。[此处原文缺失植物名称]的提取物在浓度高达1.25毫克/毫升时有效。[此处原文缺失物质名称]在浓度为0.6毫克/毫升时能够抑制和杀灭[此处原文缺失病原体名称]，但其在2.5毫克/毫升时生物膜抑制效果显著下降。
因此，该研究关于所有提取物都将表现出抗菌作用的假设得到了证实。
