Enriquez Thomas, Sassù Fabiana, Cáceres Carlos, Colinet Hervé
University of Rennes, CNRS, ECOBIO [(Ecosystèmes, biodiversité, évolution)], UMR 6553, F-35000 Rennes, France.
Insect Pest Control Section, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, Wagramerstrasse 5, PO Box 100, 1400Vienna, Austria.
Bull Entomol Res. 2021 Dec;111(6):645-657. doi: 10.1017/S0007485321000146. Epub 2021 Aug 25.
is an invasive fruit pest in Europe and America. Females lay eggs into mature fruit that larvae consume causing important losses. Sterile insect technique (SIT) is under development to control this pest. The efficiency of this technique relies on insect quality. However, during the process from mass production to field release, several steps may compromise insect quality and therefore SIT success. Shipment of sterile insects after irradiation is a key step of SIT programmes. Generally, insects are shipped as pupae and conditions during transport need to be adapted to prevent emergence before field release, while guaranteeing insect quality. To do so, transport is usually performed under low temperature, hypoxia or a combination of both. However, the impact of multiple stressors such as irradiation followed by chilling combined with hypoxia is poorly described and has not been studied in . Therefore, the aim of this study was to simulate a shipment of pupae (irradiated or not) under different conditions (chilling combined or not with hypoxia) for various durations, and to assess consequences on emerged adults. Irradiation followed by hypoxia and/or chilling only weakly altered emergence. However, 48 h of hypoxia without chilling altered the flight ability of flies whether or not they were irradiated. Conversely, when hypoxia was combined with chilling, flight ability remained similar to that of untreated flies. The use of chilling in combination with hypoxia for 48 h could be implemented as a transportation method for SIT programme on .
是欧美一种具有侵入性的水果害虫。雌虫将卵产在成熟果实中，幼虫取食果实，造成重大损失。目前正在开发不育昆虫技术（SIT）来控制这种害虫。该技术的效率依赖于昆虫质量。然而，在从大规模生产到田间释放的过程中，有几个步骤可能会损害昆虫质量，从而影响不育昆虫技术的成功。辐照后不育昆虫的运输是不育昆虫技术项目的关键步骤。一般来说，昆虫以蛹的形式运输，运输过程中的条件需要进行调整，以防止在田间释放前羽化，同时保证昆虫质量。为此，运输通常在低温、低氧或两者结合的条件下进行。然而，多种应激源（如辐照后接着低温与低氧相结合）的影响描述甚少，在[具体对象未提及]中尚未得到研究。因此，本研究的目的是模拟在不同条件下（低温与低氧结合或不结合）、不同时长运输[具体数量未提及]蛹（辐照或未辐照）的情况，并评估对羽化出的成虫的影响。辐照后接着低氧和/或低温仅对羽化产生微弱影响。然而，48小时低氧且无低温处理会改变果蝇的飞行能力，无论其是否经过辐照。相反，当低氧与低温结合时，飞行能力与未处理的果蝇相似。将低温与低氧结合使用48小时可作为[具体对象未提及]不育昆虫技术项目的一种运输方法。
