Centre International de Recherche-Développement sur l'Élevage en Zone Subhumide-CIRDES,Bobo-Dioulasso, Burkina Faso.
Laboratoire d'Entomologie Fondamentale et Appliquée, Unité de Formation et de Recherche en Sciences de la vie et de la Terre, Université Ouaga I Pr Joseph Ki Zerbo, Burkina Faso.
PLoS One. 2019 May 14;14(5):e0216802. doi: 10.1371/journal.pone.0216802. eCollection 2019.
The sterile insect technique (SIT) requires mass-rearing of the target species, irradiation to induce sexual sterility and transportation from the mass-rearing facility to the target site. Those treatments require several steps that may affect the biological quality of sterile males. This study has been carried out to evaluate the relative impact of chilling, irradiation and transport on emergence rate, flight ability and survival of sterile male Glossina palpalis gambiensis.
Chilling, irradiation and transport all affected the quality control parameters studied. The emergence rate was significantly reduced by long chilling periods and transport, i.e. from 92% at the source insectary in Burkina Faso to 78% upon arrival in Senegal. Flight ability was affected by all three parameters with 31% operational flies lost between the production facility and the destination site. Only survival under stress was not affected by any of the treatments.
The chilling period and transport were the main factors that impacted significantly the quality of sterile male pupae. Therefore, in the operational programme, the delivery of sterile male pupae was divided over two shipments per week to reduce the chilling time and improve the quality of the sterile males. Quality of the male pupae may further be improved by reducing the transport time and vibrations during transport.
无菌昆虫技术（SIT）需要大量繁殖目标物种，辐照以诱导雄性不育，并将其从大规模繁殖设施运输到目标地点。这些处理需要几个步骤，可能会影响不育雄虫的生物质量。本研究旨在评估冷藏、辐照和运输对冈比亚舌蝇不育雄虫的羽化率、飞行能力和存活率的相对影响。
冷藏、辐照和运输均影响了所研究的质量控制参数。长时间冷藏和运输显著降低了羽化率，从布基纳法索来源昆虫饲养场的 92%降至塞内加尔到达时的 78%。三种参数都影响了飞行能力，在生产设施和目的地之间有 31%的操作飞蝇丢失。只有在应激下的存活率不受任何处理的影响。
冷藏期和运输是显著影响不育雄蛹质量的主要因素。因此，在操作方案中，每周分两批交付不育雄蛹，以减少冷藏时间并提高不育雄虫的质量。通过减少运输过程中的时间和振动，可以进一步提高雄蛹的质量。
