Han Jun, Xu Wenbo, Yu Huanxi, Han Yun, Zhu Ming
Chinese Medicine Research Institute of Beijing Tcmages Pharmaceutical Co., Ltd., Beijing, China.
Nanjing Institute of Environmental Sciences, Ministry of Ecology and Environment of the People's Republic of China, Nanjing, China.
Front Plant Sci. 2024 Nov 26;15:1492723. doi: 10.3389/fpls.2024.1492723. eCollection 2024.
(Kom.) Kitag., a member of the Apiaceae family, is a perennial aromatic herb native to Northeast Asia with applications in culinary and traditional medicine. Despite its significance, most studies on have primarily focused on its phytochemical properties, with limited insights into its molecular and genomic characteristics.
This study presents the sequencing and assembly of the mitochondrial genome (mitogenome) of using second- and third-generation high-throughput sequencing technologies. Comprehensive analyses were performed on its structural organization, RNA editing sites, relative synonymous codon usage (RSCU), and repeat sequences. Comparative analyses with closely related species were also conducted.
The mitogenome exhibited a multi-branched structure, with a total length of 523,512 bp and a GC content of 43.37%. Annotation revealed 30 unique protein-coding genes, 21 tRNA genes, and three rRNA genes. Comparative analysis indicated that the mitogenome contains structural variations but shares collinear features with other Apiaceae species. We identified 618 potential RNA editing sites involving C-to-U conversions and discovered 59 homologous fragments between the mitogenome and plastome, comprising 8.13% of the mitogenome.
These results enrich the genomic database of Apiaceae, providing valuable insights into the evolutionary relationships and genetic diversity within the family.
北柴胡是伞形科的一员，是一种多年生芳香草本植物，原产于东北亚，用于烹饪和传统医学。尽管其具有重要意义，但大多数关于北柴胡的研究主要集中在其植物化学特性上，对其分子和基因组特征的了解有限。
本研究使用第二代和第三代高通量测序技术对北柴胡的线粒体基因组（线粒体基因组）进行测序和组装。对其结构组织、RNA编辑位点、相对同义密码子使用情况（RSCU）和重复序列进行了全面分析。还与近缘物种进行了比较分析。
线粒体基因组呈现多分支结构，全长523,512 bp，GC含量为43.37%。注释显示有30个独特的蛋白质编码基因、21个tRNA基因和3个rRNA基因。比较分析表明，北柴胡线粒体基因组包含结构变异，但与其他伞形科物种具有共线特征。我们鉴定出618个潜在的RNA编辑位点，涉及C到U的转换，并在线粒体基因组和质体基因组之间发现了59个同源片段，占线粒体基因组的8.13%。
这些结果丰富了伞形科的基因组数据库，为该科内的进化关系和遗传多样性提供了有价值的见解。
