Rubinstein Alejandra J, Pérez Oscar E
Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Consejo Nacional de Investigación Científica y Técnicas de la República Argentina, IQUIBICEN-CONICET, Universidad de Buenos Aires, Intendente Güiraldes, s/n, Ciudad Universitaria, Buenos Aires, CP 1428, Argentina.
Plant Foods Hum Nutr. 2024 Dec 11;80(1):7. doi: 10.1007/s11130-024-01242-2.
The objective of the present contribution was to design and characterize resveratrol (RSV) and tocopherol (TOC) loaded quinoa protein (QP) nanocomplexes, their antioxidant and antiradical functionality were also evaluated. QP intrinsic fluorescence spectra showed a quenching effect exerted by RSV and TOC, demonstrating protein-bioactive compounds interactions. Stern-Volmer and Scatchard models application confirmed the static quenching effect and allowed to obtain parameters that described the QP-RSV or QP-TOC complexation process. Isothermal titration calorimetry allowed obtaining thermodynamic parameters to describe the interaction between RSV or TOC with QP. Protein aggregates up to 40 nm in diameter were induced by bioactive compounds as detected by atomic force microscopy. QP-RSV and QP-TOC nanocomplexes showed additive effect in their antioxidant capacity. These nanocomplexes could constitute a real platform for the design of functional ingredients.
本研究的目的是设计并表征负载白藜芦醇（RSV）和生育酚（TOC）的藜麦蛋白（QP）纳米复合物，并评估其抗氧化和抗自由基功能。QP的固有荧光光谱显示出RSV和TOC产生的猝灭效应，证明了蛋白质与生物活性化合物之间的相互作用。应用Stern-Volmer和Scatchard模型证实了静态猝灭效应，并能够获得描述QP-RSV或QP-TOC络合过程的参数。等温滴定量热法能够获得描述RSV或TOC与QP之间相互作用的热力学参数。原子力显微镜检测发现，生物活性化合物可诱导形成直径达40 nm的蛋白质聚集体。QP-RSV和QP-TOC纳米复合物在抗氧化能力方面表现出加和效应。这些纳米复合物可为功能性成分的设计提供一个切实可行的平台。
