组织驻留性CD8+ T细胞作为人巨细胞病毒母乳传播中保护性免疫的介质。
Tissue-Resident CD8+ T Cells as Mediators of Protective Immunity in Breast Milk Transmission of Human Cytomegalovirus.
作者信息
Legg Shara B, Moylan David C, Kamau Neema, Latting Misty P, Devdhara Dhruv, Pati Sunil K, Crossman David K, Boppana Suresh B, Kutsch Olaf, Sabbaj Steffanie
机构信息
Department of Medicine, University of Alabama at Birmingham, Birmingham, Alabama, USA.
Department of Pediatrics, University of Alabama at Birmingham, Birmingham, Alabama, USA.
出版信息
J Infect Dis. 2025 Jun 2;231(5):1327-1335. doi: 10.1093/infdis/jiae618.
While the role of breast milk antibodies to protect infants from human cytomegalovirus (HCMV) has been investigated, the role of T-cells has received little attention. We compared the frequency of memory T-cell populations in breast milk between mothers of infants who acquired breast milk HCMV (transmitters) and those with uninfected infants (nontransmitters). Nontransmitter mothers had an increased frequency of CD8+ effector memory T-cells (Tem) in their breast milk. In addition, we describe tissue-resident memory T cells (Trm) in breast milk and demonstrate most were Tem. We present data that nontransmitter mothers have increased frequency of CD8+ Trm in their breast milk when compared to transmitters and that Trm frequency is inversely correlated with breast milk HCMV viral load. Lastly, using scRNA-seq analysis we identified a unique population of T-cells in nontransmitters and demonstrate that these cells comprise CD8+ Trm. These studies suggest that CD8+ Trm may play a role in preventing viral transmission via breast milk to infants.
虽然母乳抗体在保护婴儿免受人类巨细胞病毒（HCMV）感染方面的作用已得到研究，但T细胞的作用却很少受到关注。我们比较了母乳中感染HCMV的婴儿母亲（传播者）和未感染婴儿的母亲（非传播者）的记忆T细胞群体频率。非传播者母亲母乳中CD8 +效应记忆T细胞（Tem）的频率增加。此外，我们描述了母乳中的组织驻留记忆T细胞（Trm），并证明大多数是Tem。我们的数据表明，与传播者相比，非传播者母亲母乳中CD8 + Trm的频率增加，并且Trm频率与母乳HCMV病毒载量呈负相关。最后，使用单细胞RNA测序（scRNA-seq）分析，我们在非传播者中鉴定出独特的T细胞群体，并证明这些细胞包含CD8 + Trm。这些研究表明，CD8 + Trm可能在预防病毒通过母乳传播给婴儿方面发挥作用。
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