人巨细胞病毒（CMV）感染母亲的母乳病毒载量及母乳中CMV-pp65特异性CD8 T细胞的建立
Breast Milk Human Cytomegalovirus (CMV) Viral Load and the Establishment of Breast Milk CMV-pp65-Specific CD8 T Cells in Human CMV Infected Mothers.
作者信息
Moylan David C, Pati Sunil K, Ross Shannon A, Fowler Karen B, Boppana Suresh B, Sabbaj Steffanie
机构信息
Department of Medicine, University of Alabama at Birmingham.
Department of Pediatrics, University of Alabama at Birmingham.
出版信息
J Infect Dis. 2017 Nov 27;216(9):1176-1179. doi: 10.1093/infdis/jix457.
Abstract
The role of human cytomegalovirus (HCMV)-specific T-cell responses in breast milk of HCMV-seropositive mothers is not well defined. In these studies, we demonstrate that the frequency of cytomegalovirus (CMV)-pp65-specific T-cell responses in peripheral blood mononuclear cells (PBMCs) and breast milk cells (BMCs) is increased for CD8+ T cells in both sample sources when compared with CD4+ T cells. The frequency of pp55-specific CD8 T cells producing interferon γ (IFN-γ) alone or dual IFN-γ/granzyme rB producers is increased in breast milk compared with PBMCs. Lastly, we observed a positive correlation between breast milk viral load and the CD8 pp65-specific response, suggesting that local virus replication drives antigen-specific CD8 T cells into the breast.
摘要
人巨细胞病毒（HCMV）血清反应阳性母亲母乳中HCMV特异性T细胞反应的作用尚未明确。在这些研究中，我们证明，与CD4⁺T细胞相比，外周血单个核细胞（PBMC）和母乳细胞（BMC）中，两种样本来源的CD8⁺T细胞中巨细胞病毒（CMV）-pp65特异性T细胞反应的频率均增加。与PBMC相比，母乳中单独产生干扰素γ（IFN-γ）或同时产生IFN-γ/颗粒酶rB的pp55特异性CD8 T细胞频率增加。最后，我们观察到母乳病毒载量与CD8 pp65特异性反应之间呈正相关，这表明局部病毒复制将抗原特异性CD8 T细胞驱入乳腺。
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