Towards harmonized ecotoxicological effect assessment of micro- and nanoplastics in aquatic systems.

Micro- and nanoplastics are globally important environmental pollutants. Although research in this field is continuously improving, there are a number of uncertainties, inconsistencies and methodological challenges in the effect assessment of micro- and nanoparticles in freshwater systems. The current understanding of adverse effects is partly biased by the use of non-relevant particle types, unsuitable test setups and environmentally unrealistic dose metrics, which does not take into account realistic processes in particle uptake and consequent effects. Here we summarize the current state of the art by compiling the most recent research with the aim to highlight research gaps and further necessary steps towards more harmonized testing systems. In particular, ecotoxicological scenarios need to mirror environmentally realistic particle diversity and bioavailability. Harmonized test setups should include different uptake pathways, exposures and comparisons with natural reference particles. Effect assessments need to differentiate direct physical particle effects, such as lesions and toxicity caused by the polymer, from indirect effects, such as alterations of ambient environmental conditions by leaching, change of turbidity, food dilution and organisms' behavior. Implementation of these suggestions can contribute to harmonization and more effective, evidence-based assessments of the ecotoxicological effects of micro- and nanoplastics.