Handelin B L, Kabat D
Virology. 1985 Jan 15;140(1):183-7. doi: 10.1016/0042-6822(85)90458-1.
Two assays were developed for identifying individual cells which bear murine leukemia virus receptors: an erythrocyte rosette assay for ecotropic receptors, and an efficient immune cytotoxic assay for cells with ecotropic or amphotropic receptors. Both assays indicate that ecotropic MuLV adsorbed to its cell surface receptor only slowly becomes internalized. Furthermore, attempts to isolate murine fibroblast variants lacking these ecotropic MuLV receptors were unsuccessful, suggesting either that mutations in the receptor gene are rare (less than 10(-7) per generation) or that the receptor is required for cell viability. These assays are rapid and can be used to identify receptor-bearing cells in mixed populations, a prerequisite for molecular genetic studies.
一种用于嗜亲性受体的红细胞玫瑰花结检测法，以及一种用于具有嗜亲性或双嗜性受体的细胞的高效免疫细胞毒性检测法。两种检测方法均表明，吸附在其细胞表面受体上的嗜亲性鼠白血病病毒（MuLV）仅缓慢内化。此外，分离缺乏这些嗜亲性MuLV受体的小鼠成纤维细胞变体的尝试未成功，这表明要么受体基因突变很少见（每代少于10^(-7)），要么受体是细胞存活所必需的。这些检测方法快速，可用于鉴定混合群体中带有受体的细胞，这是分子遗传学研究的一个先决条件。
