Wang Feng, Lei Hexu, Tian Chi, Ji Yanting, Wang Fangyu, Deng Hanxin, Zhou Hongji, Chen Siyu, Zhou Yujuan, Meng Zihan, He Mengyao, Yang Shifeng, Dong Huan, Tu Ding, Wang He, Li Xian, Kaplan David L, Xia Qingyou
Integrative Science Center of Germplasm Creation in Western China (CHONGQING) Science City, Biological Science Research Center, Southwest University, Chongqing, 400715, China.
Department of Biomedical Engineering, Tufts University, Medford, MA, 02155, USA.
Adv Mater. 2025 Feb;37(7):e2414878. doi: 10.1002/adma.202414878. Epub 2024 Dec 11.
Long historical evolution and domestication endow silkworms with the super ability to synthesize and secrete massive silk proteins using silk glands. The major component of this secretion consists of silk fibroin, considered a promising biomaterial for tissue repairs and engineering. To further expand the utility of this unique protein, there is a continuing need for silk fibroin functionalization. Here, a highly-efficient Fib-H biosynthetic system is established to synthesize massive recombinant RFP in silk fibers using transgenic silkworms, which accounts for ≈7.86% of silk mass and achieves fabrication of fluorescent silk fibroin (SF) biomaterials. The universality of the Fib-H system is validated by genetic engineering glucose oxidase (GOx) functionalized silk fibers for fabricating GOx-SF hydrogels with antimicrobial activity to promote healing of infected diabetic wounds in mouse through the enzyme-catalyzed reaction of glucose to gluconic acid and HO. These findings demonstrate that the Fib-H system provides an opportunity for genetic functionalization of SF to broaden the utility of this biomaterial for a range of potential applications.
漫长的历史演变和驯化赋予了蚕利用丝腺合成和分泌大量丝蛋白的超强能力。这种分泌物的主要成分是丝素蛋白，它被认为是一种用于组织修复和工程的很有前景的生物材料。为了进一步拓展这种独特蛋白质的用途，对丝素蛋白进行功能化处理的需求一直存在。在此，建立了一种高效的Fib-H生物合成系统，利用转基因蚕在丝纤维中合成大量重组红色荧光蛋白（RFP），其占丝质量的约7.86%，并实现了荧光丝素蛋白（SF）生物材料的制备。通过基因工程使葡萄糖氧化酶（GOx）功能化丝纤维，以制备具有抗菌活性的GOx-SF水凝胶，通过葡萄糖与葡萄糖酸和过氧化氢的酶催化反应促进小鼠感染性糖尿病伤口的愈合，验证了Fib-H系统的通用性。这些发现表明，Fib-H系统为SF的基因功能化提供了一个机会，以拓宽这种生物材料在一系列潜在应用中的用途。
