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双金属银/金纳米颗粒对MCF-7乳腺癌细胞的抗肿瘤活性。

Antitumor activity of bimetallic silver/gold nanoparticles against MCF-7 breast cancer cells.

作者信息

Martínez-Sanmiguel Juan J, Zarate-Triviño Diana, García-García María Paula, García-Martín José Miguel, Mayoral Álvaro, Huttel Yves, Martínez Lidia, Cholula-Díaz Jorge L

机构信息

School of Engineering and Sciences, Tecnológico de Monterrey Av. Eugenio Garza Sada 2501 Monterrey 64849 N.L. Mexico

Laboratorio de Inmunología y Virología, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León San Nicolas de los Garza Nuevo León 66455 Mexico.

出版信息

RSC Adv. 2024 Dec 10;14(53):39102-39111. doi: 10.1039/d4ra06227b.

DOI:10.1039/d4ra06227b
PMID:39664251
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11629938/
Abstract

Breast cancer poses a global threat with rising incidence and high mortality. Conventional treatments, including chemotherapy, radiation, surgery, and immunotherapy, have side effects, such as resistance issues and adverse effects due to genetic mutations. Meanwhile, noble metal nanoparticles (NPs) synthesized using environmentally friendly methods offer alternative treatments. Bimetallic gold (Au) and silver (Ag) NPs, using natural compounds like starch as stabilizers, enhance biomedical applications, including breast cancer therapies. In this work, the optical properties, stability, and particle size of colloidal bimetallic Ag/Au NPs were analyzed using UV-visible spectroscopy and ζ-potential measurements. The structural properties of the NPs were studied by powder X-ray diffraction (PXRD), while the morphology, chemical composition and particle size were determined using scanning transmission electron microscopy (STEM). The antitumor properties of the Ag/Au NPs were analyzed on human breast cancer cells (MCF-7) using the MTT viability method, reactive oxygen species (ROS) production, and genotoxicity assays. Peripheral blood mononuclear cells (PBMCs) were used as a reference of healthy cells. UV-vis spectroscopy and EDX mapping analysis confirmed the synthesis of bimetallic Ag/Au NPs. Localized surface plasmon resonance (LSPR) absorption bands shifted from 407 nm (Ag) to 524 nm (Au) based on the chemical composition of the NPs. The Ag/Au NPs showed cytocompatibility in PBMCs and a dose-dependent anticancer effect against MCF-7 cancer cells, as well as cell death dependent on ROS production was observed, particularly in NPs with atomic compositions of 50 and 75 at% Ag. This biological activity of the bimetallic NPs was associated with genotoxic damage of 20-24% greater than that observed in the monometallic counterparts. This study demonstrated the synthesis of mono- and bimetallic Ag/Au NPs using a rapid, reproducible and environmentally friendly method, with successful biomedical application against human breast cancer MCF-7 cells.

摘要

乳腺癌发病率不断上升且死亡率高，对全球构成威胁。包括化疗、放疗、手术和免疫疗法在内的传统治疗方法都有副作用，如耐药问题以及基因突变导致的不良反应。与此同时，采用环保方法合成的贵金属纳米颗粒（NPs）提供了替代治疗方案。使用淀粉等天然化合物作为稳定剂的双金属金（Au）和银（Ag）纳米颗粒增强了生物医学应用，包括乳腺癌治疗。在这项工作中，使用紫外可见光谱和ζ电位测量分析了胶体双金属Ag/Au NPs的光学性质、稳定性和粒径。通过粉末X射线衍射（PXRD）研究了纳米颗粒的结构性质，同时使用扫描透射电子显微镜（STEM）确定了其形态、化学成分和粒径。使用MTT活力法、活性氧（ROS）生成和遗传毒性测定法，在人乳腺癌细胞（MCF-7）上分析了Ag/Au NPs的抗肿瘤特性。外周血单核细胞（PBMCs）用作健康细胞的参照。紫外可见光谱和能谱分析证实了双金属Ag/Au NPs的合成。基于纳米颗粒的化学成分，局域表面等离子体共振（LSPR）吸收带从407 nm（Ag）移至524 nm（Au）。Ag/Au NPs在PBMCs中表现出细胞相容性，对MCF-7癌细胞具有剂量依赖性抗癌作用，并且观察到细胞死亡依赖于ROS生成，特别是在原子组成为50 at%和7at% Ag的纳米颗粒中。这种双金属纳米颗粒的生物活性与遗传毒性损伤有关，比单金属对应物中观察到的遗传毒性损伤大20-24%。这项研究展示了使用快速、可重复且环保的方法合成单金属和双金属Ag/Au NPs，并成功地将其应用于对抗人类乳腺癌MCF-7细胞的生物医学领域。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7e6/11629938/3a90a771eb9a/d4ra06227b-f8.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7e6/11629938/3a90a771eb9a/d4ra06227b-f8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7e6/11629938/50a0e30215b0/d4ra06227b-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7e6/11629938/d7e9aa63aaae/d4ra06227b-f2.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7e6/11629938/ae8028015ec0/d4ra06227b-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7e6/11629938/a45bbef5812e/d4ra06227b-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7e6/11629938/a01ce5b2a509/d4ra06227b-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7e6/11629938/2692906d897b/d4ra06227b-f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7e6/11629938/3a90a771eb9a/d4ra06227b-f8.jpg

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