Kao Chengkun, Zhang Qiong, Li Jianwei, Liu Jinjin, Li Wenyu, Peng Yongzhen
National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Engineering Research Center of Beijing, Beijing University of Technology, Beijing 100124, PR China.
National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Engineering Research Center of Beijing, Beijing University of Technology, Beijing 100124, PR China.
Bioresour Technol. 2025 Feb;418:131959. doi: 10.1016/j.biortech.2024.131959. Epub 2024 Dec 10.
Enhanced nitrogen removal by hydroxylamine (NHOH) on anammox-related process recently received attention. This study investigated the impact of NHOH on the partial-denitrification/anammox (PDA) biosystem. Results show that NHOH (≤10 mg N/L) immediately induced nitrite accumulation and provided sufficient NO to anammox, achieving a 18.1 ± 4.3 % increase of nitrogen removal efficiency compared to the absence of NHOH. Long-term exposure to NHOH accelerated the functional microbial community transformation to PDA. Thauera was highly enriched (6.1 % → 26.9 %) along with Candidatus Brocadia increased in the biofilms, which mainly favor the coupling process of nitrate reduction and anammox. Although the migration mechanism of anammox and denitrifier revealed by CLSM-FISH alleviates the adverse effects of NHOH, the anammox was inhibited when NHOH exceeding 15 mg N/L through destroying the inner reduction of NO. These results suggested appropriate NHOH addition favors the synergy between denitrifying and anammox bacteria, providing a promising option for wastewater treatment.
羟胺（NHOH）对厌氧氨氧化相关过程中氮去除的强化作用近来受到关注。本研究调查了NHOH对部分反硝化/厌氧氨氧化（PDA）生物系统的影响。结果表明，NHOH（≤10 mg N/L）立即诱导亚硝酸盐积累，并为厌氧氨氧化提供足够的NO，与不添加NHOH相比，氮去除效率提高了18.1±4.3%。长期暴露于NHOH加速了功能微生物群落向PDA的转变。陶厄氏菌高度富集（从6.1%增至26.9%），同时生物膜中“Candidatus Brocadia”增加，这主要有利于硝酸盐还原与厌氧氨氧化的耦合过程。尽管CLSM-FISH揭示的厌氧氨氧化菌和反硝化菌的迁移机制减轻了NHOH的不利影响，但当NHOH超过15 mg N/L时，会通过破坏NO的内部还原而抑制厌氧氨氧化。这些结果表明，适当添加NHOH有利于反硝化菌和厌氧氨氧化菌之间的协同作用，为废水处理提供了一个有前景的选择。
