Zinc nanoparticles induced eggshell collagen membrane used for guided bone regeneration: A novel approach in rabbit models.

Conventional methods of guided bone regeneration (GBR) in bone tissue engineering frequently encounter challenges in attaining adequate antibacterial and osteogenic qualities owing to intrinsic limits. The purpose of this preclinical study was to assess the effectiveness of two distinct membranes, Healiguide® and a unique GBR membrane produced from eggshell membranes (ESM), in combination with bone graft replacement in animal models. The objective of this study was to evaluate the efficacy of a new guided bone regeneration membrane developed from eggshell membrane that incorporates zinc nanoparticles for the regeneration of bone tissue in rabbit models. Groups A (no membrane), B (new ESM GBR membrane), and C (Healiguide® membrane) were assigned to three groups, with eight male New Zealand rabbits weighing two-three kilograms each. Standardized surgical procedures were implemented, and histological analysis along with radiographic examination was used for follow-up assessments six and 12 weeks after surgery. Radiographic examination and histological sectioning revealed differences in bone density and quality between the groups. Group B showed the highest level of bone regeneration, followed by Groups C and A. Statistical analysis confirmed a significant difference between the groups (p < 0.05). The eggshell membrane showed encouraging results in the enhancement of bone regeneration and integration in rabbit models. These results suggest its potential as a viable alternative to GBR in bone tissue engineering, with promising prospects for improving clinical outcomes. However, additional studies and clinical trials are required to confirm its safety and effectiveness for medical use.