Shen Jia-Wei, Pan Po-Kai, Chen Yin-Yu, Nan Fan-Hua, Wu Yu-Sheng
Department of Aquaculture, National Pingtung University of Science and Technology, Pingtung, 912301, Taiwan.
Department of Aquaculture, National Taiwan Ocean University, Keelung, 202301, Taiwan.
Heliyon. 2024 Nov 20;10(23):e40416. doi: 10.1016/j.heliyon.2024.e40416. eCollection 2024 Dec 15.
This study evaluated the effects of hydrocolloids on Nile tilapia () using an advanced multiomics approach (transcriptome and proteome) linked with genomic isoform structure to elucidate the biofunctions of hydrocolloids. The results showed that hydrocolloids did not affect growth, as indicated by the nonsignificant differences in growth and blood biochemical indicators. Regarding the response, both intestine and liver tissues were assessed. These findings indicate that 20 % hydrocolloids enhanced cytokine expression, which may contribute to a biological function in the intestine and liver of . Genome and proteome profiles indicated that hydrocolloids upregulated the intestine and liver peroxisome proliferator-activated receptor (PPAR) signaling pathway, nucleocytoplasmic transport, steroid biosynthesis, and histidine metabolism. In contrast, co-factor biosynthesis, nucleocytoplasmic transport, tryptophan metabolism, arginine and proline metabolism, arginine biosynthesis, and ribosome activity were downregulated. These findings indicate that hydrocolloids significantly affect liver lipid and carbohydrate metabolism. Proteomics analysis revealed that hydrocolloids upregulated the PPAR signaling pathway, playing a crucial role in lipid metabolism. In summary, 20 % hydrocolloids are primarily involved in modulating the intestine and liver PPAR signaling pathway to regulate lipid metabolism.
本研究采用先进的多组学方法（转录组和蛋白质组）并结合基因组异构体结构，评估了水解胶体对尼罗罗非鱼（）的影响，以阐明水解胶体的生物功能。结果表明，水解胶体不影响生长，生长和血液生化指标的差异不显著即表明了这一点。关于反应，对肠道和肝脏组织均进行了评估。这些发现表明，20%的水解胶体可增强细胞因子表达，这可能有助于尼罗罗非鱼肠道和肝脏中的生物学功能。基因组和蛋白质组分析表明，水解胶体上调了肠道和肝脏中的过氧化物酶体增殖物激活受体（PPAR）信号通路、核质转运、类固醇生物合成和组氨酸代谢。相比之下，辅因子生物合成、核质转运、色氨酸代谢、精氨酸和脯氨酸代谢、精氨酸生物合成以及核糖体活性则被下调。这些发现表明，水解胶体显著影响肝脏脂质和碳水化合物代谢。蛋白质组学分析显示，水解胶体上调了PPAR信号通路，在脂质代谢中发挥关键作用。总之，20%的水解胶体主要参与调节肠道和肝脏的PPAR信号通路以调控脂质代谢。
